File:Calcium-signals-in-the-nucleus-accumbens-Activation-of-astrocytes-by-ATP-and-succinate-1471-2202-12-96-S1.ogv

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Calcium-signals-in-the-nucleus-accumbens-Activation-of-astrocytes-by-ATP-and-succinate-1471-2202-12-96-S1.ogv ‎(Ogg Theora video file, length 14 s, 512 × 512 pixels, 1.33 Mbps, file size: 2.19 MB)

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English: Ca2+ signalling evoked by ATP in the acute NAc slice from the rat brain. Movie of time measurement showing ATP (100 μM) application onto a Fluo-4 AM loaded acute NAc slice (300 μm). ATP was applied for 60 s (long puff) through a glass micropipette right above the tissue surface. Image acquisition frequency was 2 s in depth of 25 μm from the slice surface. Olympus FluoView300 software collected .tiff file stacks were coloured and converted to .avi file by ImageJ 1.44 (32-bit) image processing and analysis software [80]. ATP-responsive cells were counted by using an ImageJ macro developed to average stacks before the time of ATP application and to subtract this average from every stack of the 10 minute-long recording. In this way, we got a 10-minute-long movie showing only the cells that had a fluorescence intensity change.
Date
Source Molnár T, Dobolyi Á, Nyitrai G, Barabás P, Héja L, Emri Z, Palkovits M, Kardos J (2011). "Calcium signals in the nucleus accumbens: Activation of astrocytes by ATP and succinate". BMC Neuroscience. DOI:10.1186/1471-2202-12-96. PMID 21967230. PMC: 3199278.
Author Molnár T, Dobolyi Á, Nyitrai G, Barabás P, Héja L, Emri Z, Palkovits M, Kardos J
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current22:18, 20 November 201214 s, 512 × 512 (2.19 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 726 kbps Completed 00:35, 21 August 2018 30 s
Streaming 480p (VP9) 726 kbps Completed 09:44, 13 January 2024 1.0 s
VP9 360P 326 kbps Completed 00:35, 21 August 2018 26 s
Streaming 360p (VP9) 326 kbps Completed 13:05, 18 January 2024 1.0 s
VP9 240P 130 kbps Completed 00:34, 21 August 2018 13 s
Streaming 240p (VP9) 130 kbps Completed 05:27, 5 December 2023 0.0 s
WebM 360P 511 kbps Completed 22:23, 20 November 2012 13 s
Streaming 144p (MJPEG) 778 kbps Completed 17:38, 1 November 2023 1.0 s

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