File:Cytoskeletal-Components-of-an-Invasion-Machine—The-Apical-Complex-of-Toxoplasma-gondii-ppat.0020013.sv002.ogv
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Cytoskeletal-Components-of-an-Invasion-Machine—The-Apical-Complex-of-Toxoplasma-gondii-ppat.0020013.sv002.ogv (Ogg Theora video file, length 17 s, 485 × 388 pixels, 1.26 Mbps, file size: 2.51 MB)
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DescriptionCytoskeletal-Components-of-an-Invasion-Machine—The-Apical-Complex-of-Toxoplasma-gondii-ppat.0020013.sv002.ogv |
English: Cell Division in T. gondii A monolayer culture of human fibroblast cells was infected with a transgenic line of the obligate intracellular parasite T. gondii. The parasites express EGFP-IMC1 (green), a marker for the mother and daughter IMC, and mRFP-TgMORN1 (red). DIC and epifluorescence images are shown in the video. Several parasitophorous vacuoles are seen, containing two or four parasites each. The nonfluorescent host fibroblasts are seen in the DIC image only. 3D stacks of images were collected at 0.6 μ increments in z, every 10 min. The DIC image is a single plane from the middle of the stack. The epifluorescence image is a projection of the deconvolved 3D stack. The total elapsed time for the video sequence is 16 h 40 min. |
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Source | Video S2 from Hu K, Johnson J, Florens L, Fraunholz M, Suravajjala S, DiLullo C, Yates J, Roos D, Murray J (2006). "Cytoskeletal Components of an Invasion Machine—The Apical Complex of Toxoplasma gondii". PLOS Pathogens. DOI:10.1371/journal.ppat.0020013. PMID 16518471. PMC: 1383488. | ||
Author | Hu K, Johnson J, Florens L, Fraunholz M, Suravajjala S, DiLullo C, Yates J, Roos D, Murray J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 19:17, 14 November 2012 | 17 s, 485 × 388 (2.51 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Cell Division in T. gondii |
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Author | Hu K, Johnson J, Florens L, Fraunholz M, Suravajjala S, DiLullo C, Yates J, Roos D, Murray J |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | A monolayer culture of human fibroblast cells was infected with a transgenic line of the obligate intracellular parasite T. gondii. The parasites express EGFP-IMC1 (green), a marker for the mother and daughter IMC, and mRFP-TgMORN1 (red). DIC and epifluorescence images are shown in the video. Several parasitophorous vacuoles are seen, containing two or four parasites each. The nonfluorescent host fibroblasts are seen in the DIC image only. 3D stacks of images were collected at 0.6 ? increments in z, every 10 min. The DIC image is a single plane from the middle of the stack. The epifluorescence image is a projection of the deconvolved 3D stack. The total elapsed time for the video sequence is 16 h 40 min. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2006-02 |