File:Fluorescent-Protein-Stabilization-and-High-Resolution-Imaging-of-Cleared-Intact-Mouse-Brains-pone.0124650.s009.ogv
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DescriptionFluorescent-Protein-Stabilization-and-High-Resolution-Imaging-of-Cleared-Intact-Mouse-Brains-pone.0124650.s009.ogv |
English: RABV ΔG-EGFP -labeled Glia cells in the mouse brain perforant path. Video animation from a confocal recording of green fluorescence from RABVΔG-EGFP labeled neuronal structures showing neurons and glia cells in close vicinity to source cell axons running along the perforant path. Glia cells show round and bushy morphology, while the neurons are characterized by solid, compact cell bodies with clearly visible neurites. Data were recorded from the same brain hemisphere as shown in Fig 4, after 703 days in FluoClearBABB clearing solution. Voxel size of confocal data set: 0.15 * 0.15 * 0.145 microns, recorded at 12 bit resolution: After deconvolution with Huygens software, the data were converted to 8 bit grayscale for video generation with Amira software. |
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Source | S5 Video from Schwarz M, Scherbarth A, Sprengel R, Engelhardt J, Theer P, Giese G (2015). "Fluorescent-Protein Stabilization and High-Resolution Imaging of Cleared, Intact Mouse Brains". PLOS ONE. DOI:10.1371/journal.pone.0124650. PMID 25993380. PMC: 4439039. | ||
Author | Schwarz M, Scherbarth A, Sprengel R, Engelhardt J, Theer P, Giese G | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 20:34, 31 May 2015 | 12 s, 1,056 × 960 (6.43 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | RABV ΔG-EGFP -labeled Glia cells in the mouse brain perforant path. |
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Author | Schwarz M, Scherbarth A, Sprengel R, Engelhardt J, Theer P, Giese G |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Video animation from a confocal recording of green fluorescence from RABVΔG-EGFP labeled neuronal structures showing neurons and glia cells in close vicinity to source cell axons running along the perforant path. Glia cells show round and bushy morphology, while the neurons are characterized by solid, compact cell bodies with clearly visible neurites. Data were recorded from the same brain hemisphere as shown in Fig 4, after 703 days in FluoClearBABB clearing solution. Voxel size of confocal data set: 0.15 * 0.15 * 0.145 microns, recorded at 12 bit resolution: After deconvolution with Huygens software, the data were converted to 8 bit grayscale for video generation with Amira software. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-05-20 |