File:Giardia-Flagellar-Motility-Is-Not-Directly-Required-to-Maintain-Attachment-to-Surfaces-ppat.1002167.s005.ogv
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Giardia-Flagellar-Motility-Is-Not-Directly-Required-to-Maintain-Attachment-to-Surfaces-ppat.1002167.s005.ogv (Ogg Theora video file, length 42 s, 352 × 288 pixels, 96 kbps, file size: 493 KB)
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DescriptionGiardia-Flagellar-Motility-Is-Not-Directly-Required-to-Maintain-Attachment-to-Surfaces-ppat.1002167.s005.ogv |
English: Flagellar beating and lateral crest seal (wild type, alpha2-annexin dominant negative strain, anti-PF16 morpholino knockdown), TIRFM movie. Movie A shows surface contacts made by a trophozoite during attachment to a glass substrate. Note the sigmoidal ventral flagellar beat and the continuous seal made by the lateral crest of the ventral disc. Movie B is an example of the dominant negative alpha2-annexin::GFP (D122A, D275A), exhibiting a decreased ventral flagellar beat amplitude, and Movie C is an example of the PF16 knockdown exhibiting normal cell movement but a decreased flagellar beat frequency. Movies B and C show that cells form surface contacts similar to wild type, including that of the lateral crest, despite disruption of ventral flagellar function. |
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Source | Video S4 from House S, Richter D, Pham J, Dawson S (2011). "Giardia Flagellar Motility Is Not Directly Required to Maintain Attachment to Surfaces". PLOS Pathogens. DOI:10.1371/journal.ppat.1002167. PMID 21829364. PMC: 3150270. | ||
Author | House S, Richter D, Pham J, Dawson S | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 10:15, 14 November 2012 | 42 s, 352 × 288 (493 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | House S, Richter D, Pham J, Dawson S |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Flagellar beating and lateral crest seal (wild type, alpha2-annexin dominant negative strain, anti-PF16 morpholino knockdown), TIRFM movie. Movie A shows surface contacts made by a trophozoite during attachment to a glass substrate. Note the sigmoidal ventral flagellar beat and the continuous seal made by the lateral crest of the ventral disc. Movie B is an example of the dominant negative alpha2-annexin::GFP (D122A, D275A), exhibiting a decreased ventral flagellar beat amplitude, and Movie C is an example of the PF16 knockdown exhibiting normal cell movement but a decreased flagellar beat frequency. Movies B and C show that cells form surface contacts similar to wild type, including that of the lateral crest, despite disruption of ventral flagellar function. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011-08 |