File:Glia-and-Muscle-Sculpt-Neuromuscular-Arbors-by-Engulfing-Destabilized-Synaptic-Boutons-and-Shed-pbio.1000184.s003.ogv

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Glia-and-Muscle-Sculpt-Neuromuscular-Arbors-by-Engulfing-Destabilized-Synaptic-Boutons-and-Shed-pbio.1000184.s003.ogv ‎(Ogg Theora video file, length 4.3 s, 102 × 95 pixels, 236 kbps, file size: 124 KB)

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English: Active disintegration of ghost boutons into smaller structures and disappearance from the NMJ. Motorneurons were labeled with mCD8-GFP (using C380-Gal4), and imaged every 10 s for a 5-min interval. Note that one ghost bouton (center of field of view) splits into two smaller GFP+ structures, one lingers at the NMJ, while the other shifts its position dramatically and then disappears from the plane of focus. Full analysis of the Z-stack revealed that this particle had moved to a position deep within the muscle cell (unpublished data), apparently having been engulfed. A 3-D rendering of the Z-stack revealed that the presynaptic debris particles imaged remained fully within the Z-series and the changes observed were not the result of specimen drift.
Date
Source Video S1 from Fuentes-Medel Y, Logan M, Ashley J, Ataman B, Budnik V, Freeman M (2009). "Glia and Muscle Sculpt Neuromuscular Arbors by Engulfing Destabilized Synaptic Boutons and Shed Presynaptic Debris". PLOS Biology. DOI:10.1371/journal.pbio.1000184. PMID 19707574. PMC: 2724735.
Author Fuentes-Medel Y, Logan M, Ashley J, Ataman B, Budnik V, Freeman M
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Date/TimeThumbnailDimensionsUserComment
current02:20, 2 April 20174.3 s, 102 × 95 (124 KB)Revent (talk | contribs)attempt to fix transcodes
15:51, 16 November 20124.3 s, 102 × 95 (43 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 29 kbps Completed 04:46, 22 December 2023 1.0 s
WebM 360P 36 kbps Completed 11:53, 30 November 2023 0.0 s
Streaming 144p (MJPEG) 262 kbps Completed 04:52, 14 November 2023 1.0 s

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