File:Improved-molecular-toolkit-for-cAMP-studies-in-live-cells-1756-0500-4-241-S1.ogv

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Improved-molecular-toolkit-for-cAMP-studies-in-live-cells-1756-0500-4-241-S1.ogv ‎(Ogg Theora video file, length 13 s, 255 × 255 pixels, 1.39 Mbps, file size: 2.16 MB)

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English: Photoactivation of PACα mimics forskolin-induced arrest of circus cells in culture. In circus cells in cultures from the X. laevis neural tube, blebs of plasma membrane propagate around the cell circumference with a period of several minutes (Olson, 1996). Forskolin stimulation of endogenous adenylyl cyclases in control cells arrests this blebbing movement (top left panel). Blue light (488 nm) irradiation of mCherry-PACα-expressing cells mimics the effect of forskolin stimulation (top right panel). In contrast, blebbing movements are not affected in non-blue light-irradiated mCherry-PACα-expressing cells (bottom left panel) or in control cells illuminated at 488 nm (bottom right panel).
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Source Hong K, Spitzer N, Nicol X (2011). "Improved molecular toolkit for cAMP studies in live cells". BMC Research Notes. DOI:10.1186/1756-0500-4-241. PMID 21774821. PMC: 3152522.
Author Hong K, Spitzer N, Nicol X
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Date/TimeThumbnailDimensionsUserComment
current00:45, 20 November 201213 s, 255 × 255 (2.16 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 321 kbps Completed 14:29, 29 August 2018 13 s
Streaming 240p (VP9) 321 kbps Completed 06:40, 5 December 2023 1.0 s
WebM 360P 724 kbps Completed 14:46, 30 November 2023 2.0 s
Streaming 144p (MJPEG) 1.08 Mbps Completed 10:17, 15 November 2023 1.0 s

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