File:Issues-associated-with-the-use-of-phosphospecific-antibodies-to-localise-active-and-inactive-pools-1745-6150-6-4-S2.ogv
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Issues-associated-with-the-use-of-phosphospecific-antibodies-to-localise-active-and-inactive-pools-1745-6150-6-4-S2.ogv (Ogg Theora video file, length 20 s, 512 × 512 pixels, 519 kbps, file size: 1.24 MB)
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English: Supplemental Video. Z-series of images to visualise nonspecific antigens detected by anti-PTyr GSK-3 antibodies. Cells transfected with eGFP, αsh2 and βsh2 plasmids were fixed and stained using anti-GSK-3α + anti-GSK-3β monoclonal antibodies (red) and anti-GSK-3α/β pY279/216 from Merk (far red, shown as white in GFP transfected cells). Images were obtained using a confocal microscope as described in the Methods. The video shows a Z-stack of 5 optical sections from bottom (surface contact with coverslip) to top. |
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| Source | Campa V, Kypta R (2011). "Issues associated with the use of phosphospecific antibodies to localise active and inactive pools of GSK-3 in cells". Biology Direct. DOI:10.1186/1745-6150-6-4. PMID 21261990. PMC: 3039639. | ||
| Author | Campa V, Kypta R | ||
| Permission (Reusing this file) |
  This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 08:13, 20 November 2012 | 20 s, 512 × 512 (1.24 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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