File:Kinetics-of-Mosquito-Injected-Plasmodium-Sporozoites-in-Mice-Fewer-Sporozoites-Are-Injected-into-ppat.1000399.s001.ogv

English: Intravital fluorescence video showing mosquito proboscis introducing saliva into mouse ear pinna after passive immunization with fluorescent antibodies (homologous antigen and antibodies). Mosquitoes had salivary gland infection of Plasmodium berghei sporozoites. Mouse had received passive IV transfer of anti-P. berghei sporozoite monoclonal antibodies conjugated with FITC. Saliva contained sporozoites in addition to secreted, soluble P. berghei CS protein. Green density (left arrow at start of video) shows apparent immune complex formed by interaction between soluble CS protein and homologous antibodies. (Proboscis had been observed at this site just prior to initiation of time-lapse video.) Another green density (right arrow) is seen resulting from probe of another proboscis. Proboscis is visualized as orange due to autofluorescence. During 3-min, real-time duration of video, several additional green densities are formed from probosci that are out of the field of focus. Because of background density caused by FITC-labeled antibodies in tissue, relatively long exposure times of 1 sec per frame were required. Bar = 100 µm for all videos.