File:Mobilization-of-HIV-Spread-by-Diaphanous-2-Dependent-Filopodia-in-Infected-Dendritic-Cells-ppat.1002762.s016.ogv

English: Expression of HIV gag alone leads to the formation of VF. TLC cells that stably express LifeAct-mCherry (mCherry signal presented here in white to image F-actin), were transfected with TI3, a plasmid which encodes HIV Gag-iGFP equivalent to that in HIV iGFP (in green). To aid expression, psPAX2 was co-transfected, to supply WT Gag and rev in trans. To image viral filopodia, eGFP positive cells with filopodial activity were selected. Image acquisition, which the exception of acquiring the mCherry signal, was identical to that outlined for primary DC. The movie presented is representative of 20 movies derived from two independent transfections. Scale bar is presented in the left bottom corner at 15 µm. Duration of Video is 1 minute.