File:Overexpression-of-the-Wheat-Aquaporin-Gene-TaAQP7-Enhances-Drought-Tolerance-in-Transgenic-Tobacco-pone.0052439.s005.ogv

English: The oocytes swelling in TaAQP7-expressing oocytes. The defolliculated oocytes were injected with 50 nl of cRNA (1 mg/ml) or water using as negative control and then the oocytes were incubated at 18°C for 48 h in ND96 solution supplemented with 10 µg/ml penicillin and 10 µg/ml streptomycin. The oocytes were transferred to five-fold diluted ND96 solution, and then the oocytes volume were monitored at room temperature with a microscope video system by taking digital images at 30s intervals. The upper (yellow) is the control and the lower (blue) is the oocytes injected with cRNA of TaAQP7. Three biological experiments were performed, which produced similar results.