File:Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s017.ogv

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Pseudomonas-aeruginosa-ExoT-Induces-Atypical-Anoikis-Apoptosis-in-Target-Host-Cells-by-Transforming-ppat.1004934.s017.ogv ‎(Ogg multiplexed audio/video file, Theora/Vorbis, length 3.3 s, 320 × 152 pixels, 196 kbps overall, file size: 80 KB)

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English: The SH3 domain of CrkI must be functional for CrkI to mediate ExoT cytotoxicity. Crk-/- cells were transfected with the pCrkI/R38K,W170K-GFP vector on consecutive days to increase transfection efficiency. 24 hr after final transfection, transfected cells were infected with PA103ΔexoU (ΔU), PA103∆exoU/exoT(R149K) (∆U/T(G-A+)), or the T3SS mutant PA103 pscJ::Tn5 (pscJ) at MOI ~10. Video images were captured every 15 min at 200× magnification. Cellular uptake of propidium iodide (PI), an impermeant nuclear dye, identifies dying cells (red). For clarity, phase panels were excluded from the movie. Note that transfected cells (green) do not pick up PI
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Source S8 Movie from Wood S, Goldufsky J, Shafikhani S (2015). "Pseudomonas aeruginosa ExoT Induces Atypical Anoikis Apoptosis in Target Host Cells by Transforming Crk Adaptor Protein into a Cytotoxin". PLOS Pathogens. DOI:10.1371/journal.ppat.1004934. PMID 26020630. PMC: 4447348.
Author Wood S, Goldufsky J, Shafikhani S
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Date/TimeThumbnailDimensionsUserComment
current20:27, 10 June 20153.3 s, 320 × 152 (80 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P Not ready Unknown status
Streaming 240p (VP9) 95 kbps Completed 01:22, 16 December 2023 1.0 s
WebM 360P 501 kbps Completed 06:55, 2 December 2023 1.0 s
Streaming 144p (MJPEG) 1.27 Mbps Completed 18:43, 20 November 2023 1.0 s
Stereo (Opus) 5 kbps Completed 23:59, 22 November 2023 2.0 s
Stereo (MP3) 131 kbps Completed 18:43, 20 November 2023 1.0 s

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