File:Selective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s005.ogv

English: GFP-Salmonella containing human primary B cells show invasive behavior in a monolayer of CD40L-expressing 3T3 fibroblasts. A co-culture of living primary human B cells infected with GFP-expressing Salmonella and CD40L-expressing 3T3 cells was imaged using widefield fluorescence microscopy at 37°C. Depicted is the GFP signal projected on the transmission image with images taken every 30 min. The B cells repeatedly move under and over the monolayer. Arrows indicate the B cell, white: above the monolayer, black: under the monolayer. This Movie demonstrates the improved survival of B cells when co-cultured with CD40L expressing 3T3 cells. Also, excretion of the bacterium towards the end of the Movie (best visible at 12 h) is observed although the Salmonella is still contacting the B cell. Total duration: 15 hours.