File:Selective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s005.ogv
Selective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s005.ogv (Ogg Theora video file, length 15 s, 650 × 500 pixels, 473 kbps, file size: 866 KB)
Captions
Summary
[edit]DescriptionSelective-Infection-of-Antigen-Specific-B-Lymphocytes-by-Salmonella-Mediates-Bacterial-Survival-and-pone.0050667.s005.ogv |
English: GFP-Salmonella containing human primary B cells show invasive behavior in a monolayer of CD40L-expressing 3T3 fibroblasts. A co-culture of living primary human B cells infected with GFP-expressing Salmonella and CD40L-expressing 3T3 cells was imaged using widefield fluorescence microscopy at 37°C. Depicted is the GFP signal projected on the transmission image with images taken every 30 min. The B cells repeatedly move under and over the monolayer. Arrows indicate the B cell, white: above the monolayer, black: under the monolayer. This Movie demonstrates the improved survival of B cells when co-cultured with CD40L expressing 3T3 cells. Also, excretion of the bacterium towards the end of the Movie (best visible at 12 h) is observed although the Salmonella is still contacting the B cell. Total duration: 15 hours. |
||
Date | |||
Source | Video S4 from Souwer Y, Griekspoor A, de Wit J, Martinoli C, Zagato E, Janssen H, Jorritsma T, Bar-Ephraïm Y, Rescigno M, Neefjes J, van Ham S (2012). "Selective Infection of Antigen-Specific B Lymphocytes by Salmonella Mediates Bacterial Survival and Systemic Spreading of Infection". PLOS ONE. DOI:10.1371/journal.pone.0050667. PMID 23209805. PMC: 3510171. | ||
Author | Souwer Y, Griekspoor A, de Wit J, Martinoli C, Zagato E, Janssen H, Jorritsma T, Bar-Ephraïm Y, Rescigno M, Neefjes J, van Ham S | ||
Permission (Reusing this file) |
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 21:40, 6 December 2012 | 15 s, 650 × 500 (866 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | |
---|---|
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012 |