File:Tyrosine-Binding-Protein-Sites-Regulate-the-Intracellular-Trafficking-and-Processing-of-Amyloid-pone.0161445.s006.ogv

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Tyrosine-Binding-Protein-Sites-Regulate-the-Intracellular-Trafficking-and-Processing-of-Amyloid-pone.0161445.s006.ogv ‎(Ogg Theora video file, length 12 s, 466 × 463 pixels, 1.99 Mbps, file size: 2.83 MB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary[edit]

Description
English: APP bearing the dephosphomimetic (S711A) mutation traffics to lysosomes. SN56 cells were transiently transfected with βAPP S711A-paGFP (green), LAMP1-mRFP (lysosome marker, red), and GalT-CFP (Golgi marker, blue). APP was photo-activated in the Golgi (blue) with 405nm light, alternating with imaging for 15 minutes (indicated by green word ‘Photo-activating’). The white circles appearing over the Golgi denote the initial ROIs for βAPP-paGFP photoactivation. Cells were then chased by imaging every 30 seconds for the indicated time. This movie has been intentionally cropped to focus on the trafficking around the Golgi.
Date
Source S5 Video from Tam J, Cobb M, Seah C, Pasternak S (2016). "Tyrosine Binding Protein Sites Regulate the Intracellular Trafficking and Processing of Amyloid Precursor Protein through a Novel Lysosome-Directed Pathway". PLOS ONE. DOI:10.1371/journal.pone.0161445. PMID 27776132. PMC: 5077117.
Author Tam J, Cobb M, Seah C, Pasternak S
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 4.0 International license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current09:00, 31 January 201712 s, 466 × 463 (2.83 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

You cannot overwrite this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 360P 421 kbps Completed 11:08, 24 October 2018 9.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 228 kbps Completed 11:08, 24 October 2018 7.0 s
Streaming 240p (VP9) 228 kbps Completed 04:32, 21 December 2023 1.0 s
WebM 360P 524 kbps Completed 09:01, 31 January 2017 6.0 s
Streaming 144p (MJPEG) 489 kbps Completed 02:30, 11 November 2023 1.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Tyrosine-Binding-Protein-Sites-Regulate-the-Intracellular-Trafficking-and-Processing-of-Amyloid-pone.0161445.s006.ogv&oldid=463584312"