File:Visualizing-fusion-of-pseudotyped-HIV-1-particles-in-real-time-by-live-cell-microscopy-1742-4690-6-84-S5.ogv
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Visualizing-fusion-of-pseudotyped-HIV-1-particles-in-real-time-by-live-cell-microscopy-1742-4690-6-84-S5.ogv (Ogg Theora video file, length 3.2 s, 164 × 120 pixels, 321 kbps, file size: 125 KB)
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DescriptionVisualizing-fusion-of-pseudotyped-HIV-1-particles-in-real-time-by-live-cell-microscopy-1742-4690-6-84-S5.ogv |
English: Movie displaying an individual fusion event indicated by color separation, corresponding to still images in Figure 5A. Env.YFP (green) and MA.mCherry (red) labeled particles were coated onto a glass coverslip, and DFJ-8 cells were allowed to settle onto the virus particles. Image acquisition with a time resolution of 0.76 frames/sec was started at the time point of cell attachment to the coverslip. The video shows a section of the movie covering 38 sec and is displayed at a speed of 10 frames per second. The particle of interest is indicated by a white circle. While the Env.YFP signal vanished within 15 sec after virus-cell contact, the label of the MA domain remained punctated during the remaining period of observation. Still images of the video are shown in Figure 5A. |
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Date | |||
Source | Koch P, Lampe M, Godinez W, Müller B, Rohr K, Kräusslich H, Lehmann M (2009). "Visualizing fusion of pseudotyped HIV-1 particles in real time by live cell microscopy". Retrovirology. DOI:10.1186/1742-4690-6-84. PMID 19765276. PMC: 2762461. | ||
Author | Koch P, Lampe M, Godinez W, Müller B, Rohr K, Kräusslich H, Lehmann M | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 09:35, 20 November 2012 | 3.2 s, 164 × 120 (125 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 5 |
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Author | |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Movie displaying an individual fusion event indicated by color separation, corresponding to still images in Figure 5A. Env.YFP (green) and MA.mCherry (red) labeled particles were coated onto a glass coverslip, and DFJ-8 cells were allowed to settle onto the virus particles. Image acquisition with a time resolution of 0.76 frames/sec was started at the time point of cell attachment to the coverslip. The video shows a section of the movie covering 38 sec and is displayed at a speed of 10 frames per second. The particle of interest is indicated by a white circle. While the Env.YFP signal vanished within 15 sec after virus-cell contact, the label of the MA domain remained punctated during the remaining period of observation. Still images of the video are shown in Figure 5A. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |