File:A-Computational-Framework-for-Ultrastructural-Mapping-of-Neural-Circuitry-pbio.1000074.sv003.ogv

English: QuickTime Movie of the MosaicBuilder I nterface See Figure 14. Here we zoom to the synaptic level with structural markups (captured in real-time by iShowU, http://shinywhitebox.com). Tiles from a vertical section of rabbit retina prepared for assessment of CN capture parameters were captured at 5,000× with SerialEM, pipelined through ir-translate and ir-refine-grid into a large mosaic and visualized in MosaicBuilder for synaptic markup. The movie shows slider-based zooming of the dataset from a moderate scale up to synaptic level, first to a characteristic AC → GC synapse in the OFF sublamina. The synapse is marked by a red pin in the synaptic cleft and an arrow denoting polarity. These markup metadata are recorded in an *.xml export. The field is then zoomed out and repositioned to show a characteristic AC → ON cone BC synapse. Two blurring events in zooming represent the transitions in resolution in the image pyramid scheme.