File:A-Novel-Cervical-Spinal-Cord-Window-Preparation-Allows-for-Two-Photon-Imaging-of-T-Cell-video 1.ogv

English: Arrest of activated 2D2 GFP CD4+ T cells within inflamed cervical spinal cord post-capillary venules during experimental autoimmune encephalomyelitis (EAE). Active EAE was induced as described in Section “Methods.” Laminectomy was performed (described in step 15 of the procedures) on day 14 post-immunization when the mouse showed onset of disease. In vitro activated CD4+ T cells from “2D2-GFP”-mice were injected via a carotid catheter before 2P-IVM imaging. Blood vessels were labeled by injection of Alexa Fluor 594 conjugated anti-endoglin antibody. GFP (green, CD4+ T cells) and anti-endoglin (red, blood vessels) were excited at 800 nm. A x–y–t time-lapse sequence of a 400 μm × 400 μm scan field at a depth of 59–91 µm and 9 z-stacks with 4 μm spacing shows the arrest of CD4+ T cells within post-capillary venules of the cervical spinal cord. Time is shown in minutes and seconds. Scale bar: 50 μm. This video is the source video of which images shown in Figure 5 were taken.