File:A-Secreted-BMP-Antagonist-Cer1-Fine-Tunes-the-Spatial-Organization-of-the-Ureteric-Bud-Tree-during-pone.0027676.s010.ogv

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English: Cer1 expression changes the overall pattern of ureteric bud branching when compared to controls. The kidney primordial were prepared at E11.5 and subjected to organ culture for 120 hrs. Ureteric was visualized by genetic means by yellow fluorescent protein that was activated from the floxed Rosa26 locus as a result of HoxB7Cre recombination. Analysis of the time-lapse recordings reveal that Cer1 gain of function in the ureteric bud shifts the mode of ureteric bud branching from a difurgation type towards the trifurgation one (see also Figure S4). C, D) Later Cer1 over expressing kidneys have a tendency to develop also lateral side branches not that typical in the control. As a result the overall pattern formation of the ureteric bud of the kidneys that were prepared from the Cer1 kidneys appears different from the control.
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Source Movie S1 from Chi L, Saarela U, Railo A, Prunskaite-Hyyryläinen R, Skovorodkin I, Anthony S, Katsu K, Liu Y, Shan J, Salgueiro A, Belo J, Davies J, Yokouchi Y, Vainio S (2011). "A Secreted BMP Antagonist, Cer1, Fine Tunes the Spatial Organization of the Ureteric Bud Tree during Mouse Kidney Development". PLOS ONE. DOI:10.1371/journal.pone.0027676. PMID 22114682. PMC: 3219680.
Author Chi L, Saarela U, Railo A, Prunskaite-Hyyryläinen R, Skovorodkin I, Anthony S, Katsu K, Liu Y, Shan J, Salgueiro A, Belo J, Davies J, Yokouchi Y, Vainio S
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