File:A-sensitive-and-bright-single-cell-resolution-live-imaging-reporter-of-Wntß-catenin-signaling-in-1471-213X-10-121-S2.ogv

English: Tracking H2B-GFP reporter expressing cells in the visceral endoderm of TCF/Lef:H2B-GFP embryos. Registered rendered images focusing on a TCF/Lef:H2B-GFP Tg/+ embryo which remained in the field of view for the duration of the 3D time-lapse experiment depicted in the previous movie (A-D). Duration of time-lapse experiment was 9 hours 46 minutes and 21 seconds (t = 9:46:21). Individual cells identified by H2B-GFP nuclear-labeling were color-coded (colored spheres depict individual cells) and tracked using the spots function in Imaris (Bitplane, Inc.). The movie consists of 37 frames each depicting one time-point. The first frame (t = 0) depicts the initial state, with 4 non-dividing cells used as a reference for both position and distance. At frame 15 (t = 3:48:02), the first tracked cell division occurs below the bottom reference cell. Thereafter, tracked cell divisions occur in frames 21, 23, 25, 27, 28, 31, 33 and 37. Throughout the observation period, nearest-neighbor relationships are preserved, despite the substantial growth of the embryo. The change in the angle between the reference cells, as well as the distance between them, suggests that circumferential (lateral) expansion of the embryo is greater than the proximal-distal (longitudinal) growth. Scale bar: 30 μm depicted in lower left.