File:Actomyosin-dependent-dynamic-spatial-patterns-of-cytoskeletal-components-drive-mesoscale-podosome-ncomms13127-s13.ogv

English: Supplementary Movie 12 Podosome cluster formation on a patterned surface. DCs were transfected with LifeAct-GFP to visualize F-actin and seeded on a scratched glass coverslip. Podosome dissolution was induced by addition of 10 μM nocodazole, which was washed away when the dissolution was complete. Imaging was performed at a confocal microscope with 15 second frame intervals at 37°C. The time series (288 frames) was split into 3 overlapping series of 100 frames, which were subjected to twSTICS analysis. The LifeAct-GFP Movie (right) is shown as a moving average of 10 frames to allow direct comparison with the vector maps (bottom), which are calculated over 10 frames. The arrows indicate direction of flow and the size and colour coding of the vectors are representative of the flow magnitude. Two cells are shown consecutively. Scale bar represents 10 μm.