File:Actomyosin-dependent-dynamic-spatial-patterns-of-cytoskeletal-components-drive-mesoscale-podosome-ncomms13127-s19.ogv

English: Supplementary Movie 18 Actin polymerization and network integrity are essential for actin flux in podosome clusters. DCs were co-transfected with LifeAct-RFP (top) and talin-GFP (bottom) and seeded in a glass bottom dish. Imaging was performed at a confocal microscope with 15 second frame intervals, after 50 frames 2.5 μg ml-1 CytoD was added and imaging was continued up to 100 frames. Time series for both channels were subjected to twSTICS analysis. The original Movies are shown as a moving average of 10 frames to allow direct comparison with the vector maps, which are calculated over 10 frames. The arrows indicate direction of flow and the size and colour coding of the vectors are representative of the flow magnitude. The vector map is plotted onto the immobile filtered version of the time series. Scale bar represents 10 μm.