File:Ameobal-Pathogen-Mimivirus-Infects-Macrophages-through-Phagocytosis-ppat.1000087.s004.ogv
Ameobal-Pathogen-Mimivirus-Infects-Macrophages-through-Phagocytosis-ppat.1000087.s004.ogv (Ogg Theora video file, length 36 s, 320 × 240 pixels, 486 kbps, file size: 2.1 MB)
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DescriptionAmeobal-Pathogen-Mimivirus-Infects-Macrophages-through-Phagocytosis-ppat.1000087.s004.ogv |
English: 3D reconstruction of APMV engulfment by macrophages. RAW 264.7 macrophages were incubated with APMV for 5 min. F-actin distribution was studied with Alexa Fluor 488-phalloidin (green) and APMV localization was determined by immunofluorescence (red). Using a laser-scanning microscope, a series of 22 pictures (Z section) in each fluorescent channel were captured before transfer into Imaris software and 3D reconstruction. The top and bottom of cells were removed with Imaris software to improve the visual quality of the 3D reconstruction. |
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Source | Video S1 from Ghigo E, Kartenbeck J, Lien P, Pelkmans L, Capo C, Mege J, Raoult D (2008). "Ameobal Pathogen Mimivirus Infects Macrophages through Phagocytosis". PLOS Pathogens. DOI:10.1371/journal.ppat.1000087. PMID 18551172. PMC: 2398789. | ||
Author | Ghigo E, Kartenbeck J, Lien P, Pelkmans L, Capo C, Mege J, Raoult D | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 12:03, 17 November 2012 | 36 s, 320 × 240 (2.1 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Ghigo E, Kartenbeck J, Lien P, Pelkmans L, Capo C, Mege J, Raoult D |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | 3D reconstruction of APMV engulfment by macrophages. RAW 264.7 macrophages were incubated with APMV for 5 min. F-actin distribution was studied with Alexa Fluor 488-phalloidin (green) and APMV localization was determined by immunofluorescence (red). Using a laser-scanning microscope, a series of 22 pictures (Z section) in each fluorescent channel were captured before transfer into Imaris software and 3D reconstruction. The top and bottom of cells were removed with Imaris software to improve the visual quality of the 3D reconstruction. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2008-06 |