File:Aurora-A-drives-early-signalling-and-vesicle-dynamics-during-T-cell-activation-ncomms11389-s7.ogv
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[edit]DescriptionAurora-A-drives-early-signalling-and-vesicle-dynamics-during-T-cell-activation-ncomms11389-s7.ogv |
English: Supplementary Movie 6 Tracking of EB3-GFP-decorated, growing TIPs at the IS in Aurora A-deficient CD4+ T cells. CD4+ T cells isolated from Aurka(lox/lox); RERT(ert/ert) treated with tamoxifen and transfected with EB3-GFP were allowed to settle on stimulatory anti-CD3/CD28-coated surfaces and recorded under a TIRFm, at a 150 nm of penetrance upon excitation with a 488 nm laser. Images were taken every 300 ms. MLN8237 or vehicle was present in the imaging medium. Imaris Software was used to recognize fluorescence corresponding to the decorated tips and to calculate the trajectories and growing speed of the tips. Movie was mounted at 30 fps. |
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Source | Video file from Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F (2016). "Aurora A drives early signalling and vesicle dynamics during T-cell activation". Nature Communications. DOI:10.1038/ncomms11389. PMID 27091106. PMC: 4838898. | ||
Author | Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 06:21, 28 October 2016 | 20 s, 1,024 × 768 (2.44 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Supplementary Movie 6 |
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Author | Blas-Rus N, Bustos-Morán E, Pérez de Castro I, de Cárcer G, Borroto A, Camafeita E, Jorge I, Vázquez J, Alarcón B, Malumbres M, Martín-Cófreces N, Sánchez-Madrid F |
Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Tracking of EB3-GFP-decorated, growing TIPs at the IS in Aurora A-deficient CD4+ T cells. CD4+ T cells isolated from Aurka(lox/lox); RERT(ert/ert) treated with tamoxifen and transfected with EB3-GFP were allowed to settle on stimulatory anti-CD3/CD28-coated surfaces and recorded under a TIRFm, at a 150 nm of penetrance upon excitation with a 488 nm laser. Images were taken every 300 ms. MLN8237 or vehicle was present in the imaging medium. Imaris Software was used to recognize fluorescence corresponding to the decorated tips and to calculate the trajectories and growing speed of the tips. Movie was mounted at 30 fps. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2016-04-19 |
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19 April 2016
application/ogg
895f0ccf94e2da716558789458b8637308f300fd
2,559,693 byte
19.95 second
768 pixel
1,024 pixel
Categories:
- Videos of CD4-positive T-lymphocytes
- CD antigens
- CD3 antigens
- T-lymphocyte differentiation antigens
- Aurora kinase A
- Cytoplasmic vesicles
- Gene expression regulation
- Immunological synapses
- Interleukin-2 receptor alpha subunit
- C-type lectins
- Lymphocyte activation
- Lymphocyte specific protein tyrosine kinase p56
- Transgenic mice
- Protein kinase inhibitors
- T-cell antigen receptors
- Videos of signal transduction