File:BarrBodyBMC Biology2-21-Fig1.jpg

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Deutsch: Zellkerne aus normalen und verschiedenen krankhaft veränderten menschlichen, weiblichen Fibroblasten wurden mit dem DNA-Farbstoff Dapi (blau) angefärbt, um das Barr Körperchen, also das inaktive X-Chromosom anzufärben (Pfeil). Außerdem wurden verschiedene Histonmodifikationen beziehungsweise eine Sonderform eines Histons (macroH2A) mit Antikörpern nachgewiesen (grün). In A sind solche nachgewiesen, die am Barr-Körperchen nicht vorkommen, während jene in B dort besonders angereichert sind.
English: Original figure legend: The interphase inactive X in normal and mutant cells: histone modification and macroH2A1 association. Photomicrograph examples of normal, ICF, and Rett fibroblasts that were FITC-labeled using antisera to various modified histones. Arrows point to sex chromatin on DAPI-stained cells, and to the corresponding sex chromatin site in the FITC-labeled photo. A. Normal, ICF, and Rett fibroblasts FITC-labeled using antisera to acetylated histone H4 (acH4), acetylated histone H3 (acH3), and dimethylated K4 histone H3 (meK4H3). Note that the sex chromatin body is not stained by these antibodies and appears as a hole or a gap that occasionally contains a prominent dot (see insets). This FITC-stained dot appears to correspond to the DXZ4 domain, as described in the text. B. Normal and ICF fibroblasts labeled with antibody to dimethylated K9 histone H3 (meK9H3) and macrohistone H2A1 (macroH2A).
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Source BMC Biology 2004, 2:21 doi:10.1186/1741-7007-2-21
Author Stanley M Gartler, Kartik R Varadarajan, Ping Luo, Theresa K Canfield, Jeff Traynor, Uta Francke and R Scott Hansen
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