File:Control-of-Uterine-Microenvironment-by-Foxp3+-Cells-Facilitates-Embryo-Implantation-Movie 3.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
Size of this JPG preview of this OGG file: 800 × 409 pixels. Other resolutions: 320 × 163 pixels | 640 × 327 pixels | 1,496 × 764 pixels.
Original file (Ogg Theora video file, length 13 s, 1,496 × 764 pixels, 6.42 Mbps, file size: 9.97 MB)
File information
Structured data
Captions
Summary
[edit]DescriptionControl-of-Uterine-Microenvironment-by-Foxp3+-Cells-Facilitates-Embryo-Implantation-Movie 3.ogv |
English: Metestrus: shows a video from a 3D reconstruction of live 2-photon microscopy showing almost undetectable green Tregs within the uterus. Animals were anesthetized by i.p. injection of ketamine and xylazine, 120 or 16 μg/g of mouse weight, respectively, and kept on a heating pad at 37°C. One of the uterine horns was carefully exposed and 0.1 M caffeine (Sigma-Aldrich, USA) was applied to decrease uterine contractions. For maternal blood visualization, animals were intravenously injected with 100 μl of Rhodamine B isothiocyanate-Dextran (RhoB-Dex) 70,000 KDa (Sigma-Aldrich, Inc., USA) before acquiring images in a multiphoton laser scanning microscope (MPLSM). We used a Prairie Ultima 2-photon microscope (Prairie Technologies, Inc.). The microscope was equipped with a Chameleon Ti:Sapphire laser (Coherent, Inc., USA), four top PMTs for simultaneous up to four channel acquisitions and a 20× water immersion objective (Olympus, Inc., USA). The laser was tuned to 880 nm to allow for concomitant excitation of RhoB-Dex and GFP+-Treg. The wavelength emission for RhoB-Dex and GFP is 590 and 509 nm, respectively. Sequential images were acquired for observation of Treg in the uterus. For analysis of uterine Treg images, we have developed our own software algorithms based on endogenous tissue markers information (e.g., location of blood vessels) from consecutive z-stacks acquisitions for stabilization of Treg movies. Once the images were stabilized, we used Imaris software (Bitplane AG, Inc.) for reconstruction of three-dimensional models in order to determine distribution in the uterus. |
||
Date | |||
Source | Supplementary Movie S3 from Teles A, Schumacher A, Kuhnle M, Linzke N, Thuere C, Reichardt P, Tadokoro C, Hammerling G, Zenclussen A (2013). "Control of Uterine Microenvironment by Foxp3+ Cells Facilitates Embryo Implantation". Frontiers in Immunology. DOI:10.3389/fimmu.2013.00158. PMID 23801995. PMC: 3689029. | ||
Author | Teles A, Schumacher A, Kuhnle M, Linzke N, Thuere C, Reichardt P, Tadokoro C, Hammerling G, Zenclussen A | ||
Permission (Reusing this file) |
This file is licensed under the Creative Commons Attribution 3.0 Unported license.
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 22:28, 29 June 2013 | 13 s, 1,496 × 764 (9.97 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Teles A, Schumacher A, Kuhnle M, Linzke N, Thuere C, Reichardt P, Tadokoro C, Hammerling G, Zenclussen A |
---|---|
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-06-05 |