File:Distinct-Dynamics-of-Endocytic-Clathrin-Coated-Pits-and-Coated-Plaques-pbio.1000191.s011.ogv

English: Clathrin-coat dynamics at the adherent surface of HeLa cells depleted of AP-2. Time-series images of clathrin in HeLa cells whose AP-2s were depleted by using sRNAi specific for μ2. The images were acquired by alternating two channels in order to register the fluorescent signals for the stably expressed σ2-EGFP (left panel) and the transiently expressed Tomato-LCa (right panel). The video was acquired by spinning disk confocal microscopy at 10-s intervals and 200- and 100-ms exposure times, respectively. The video shows 50 frames (500 s). The mobile clathrin-coated structures in the cytosol and perinuclear regions have the usual characteristics of endosome- or TGN-associated coats containing AP-1, AP-3, GGAs, or Hrs.