File:F-actin-rich-contractile-endothelial-pores-prevent-vascular-leakage-during-leukocyte-diapedesis-ncomms10493-s7.ogv

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Original file ‎(Ogg Theora video file, length 5.5 s, 1,428 × 373 pixels, 4.02 Mbps, file size: 2.63 MB)

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English: Supplementary Movie 6 Endothelial F-actin and VE-cadherin dynamics during neutrophil diapedesis under physiological flow conditions (0.8dyne per cm2). Real-time confocal recordings of transmigrating neutrophils through ECs expressing GFP-tagged Lifeact (green) showed increased F-actin assembly around endothelial pores. Neutrophils tend to breech junctions containing low amount of VE-cadherin (red) molecules. Images were acquired by confocal laser scanning microscopy (LSM510/Meta; Carl Zeiss Micro-Imaging) using a 63x 1.4 NA oil immersion objective. Frames were taken every 14 s for 7 minutes.
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Source Video file from Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J (2016). "F-actin-rich contractile endothelial pores prevent vascular leakage during leukocyte diapedesis through local RhoA signalling". Nature Communications. DOI:10.1038/ncomms10493. PMID 26814335. PMC: 4737874.
Author Heemskerk N, Schimmel L, Oort C, van Rijssel J, Yin T, Ma B, van Unen J, Pitter B, Huveneers S, Goedhart J, Wu Y, Montanez E, Woodfin A, van Buul J
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Date/TimeThumbnailDimensionsUserComment
current13:01, 29 October 20165.5 s, 1,428 × 373 (2.63 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 961 kbps Completed 09:18, 26 August 2018 4.0 s
Streaming 720p (VP9) 962 kbps Completed 05:34, 22 March 2024 1.0 s
VP9 480P 421 kbps Completed 09:18, 26 August 2018 4.0 s
Streaming 480p (VP9) 422 kbps Completed 04:05, 7 February 2024 1.0 s
VP9 360P 242 kbps Completed 09:18, 26 August 2018 3.0 s
Streaming 360p (VP9) 243 kbps Completed 07:39, 14 March 2024 0.0 s
VP9 240P 136 kbps Completed 09:18, 26 August 2018 3.0 s
Streaming 240p (VP9) 137 kbps Completed 04:43, 21 December 2023 0.0 s
WebM 360P 514 kbps Completed 13:45, 29 October 2016 4.0 s
Streaming 144p (MJPEG) 373 kbps Completed 20:53, 10 November 2023 1.0 s

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