File:High-Sensitivity-Real-Time-Imaging-of-Dual-Protein-Protein-Interactions-in-Living-Subjects-Using-pone.0005868.s009.ogv
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High-Sensitivity-Real-Time-Imaging-of-Dual-Protein-Protein-Interactions-in-Living-Subjects-Using-pone.0005868.s009.ogv (Ogg Theora video file, length 13 s, 256 × 256 pixels, 468 kbps, file size: 743 KB)
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[edit]DescriptionHigh-Sensitivity-Real-Time-Imaging-of-Dual-Protein-Protein-Interactions-in-Living-Subjects-Using-pone.0005868.s009.ogv |
English: Three mRNAs of CBRN-Smad2, Smad4-McLuc1 and Venus were microinjected into two diagonal blastomeres of the 2-cell embryo. The embryo was set on a glass dish and dipped in a solution including 1 mM D-luciferin. The embryonic development was monitored over 12 hours under the fluorescence and bioluminescence upright microscope. Obtained digital images of bioluminescence (pseudocolor) were overlaid with the images of fluorescence (gray) at each time. |
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Source | Movie S2 from Hida N, Awais M, Takeuchi M, Ueno N, Tashiro M, Takagi C, Singh T, Hayashi M, Ohmiya Y, Ozawa T (2009). "High-Sensitivity Real-Time Imaging of Dual Protein-Protein Interactions in Living Subjects Using Multicolor Luciferases". PLOS ONE. DOI:10.1371/journal.pone.0005868. PMID 19536355. PMC: 2697115. | ||
Author | Hida N, Awais M, Takeuchi M, Ueno N, Tashiro M, Takagi C, Singh T, Hayashi M, Ohmiya Y, Ozawa T | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 01:50, 17 November 2012 | 13 s, 256 × 256 (743 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Hida N, Awais M, Takeuchi M, Ueno N, Tashiro M, Takagi C, Singh T, Hayashi M, Ohmiya Y, Ozawa T |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Three mRNAs of CBRN-Smad2, Smad4-McLuc1 and Venus were microinjected into two diagonal blastomeres of the 2-cell embryo. The embryo was set on a glass dish and dipped in a solution including 1 mM D-luciferin. The embryonic development was monitored over 12 hours under the fluorescence and bioluminescence upright microscope. Obtained digital images of bioluminescence (pseudocolor) were overlaid with the images of fluorescence (gray) at each time. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2009 |