File:Hypoxia-Transiently-Sequesters-Mps1-and-Polo-to-Collagenase-Sensitive-Filaments-in-Drosophila-pone.0007544.s003.ogv

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Original file ‎(Ogg Theora video file, length 6.5 s, 960 × 540 pixels, 542 kbps, file size: 431 KB)

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English: Heat shock does not induce GFP-Polo localization to filaments. A stage 13 GFP-Polo oocyte was heated to 37°C 18 minutes before live imaging commenced. This did not induce GFP localization to filaments. Seven other oocytes on the same slide were also monitored but not live-imaged; all exhibited similar localization before, during and after imaging as the oocyte shown. CO2 was turned on after the first frame, and the filaments appeared shortly thereafter, indicating the cell was still capable of sequestering GFP-Polo to filaments at the elevated temperature. After the eighth time point (5:15), filming was paused while the other seven oocytes were examined. After filming resumed (12:22), the CO2 was turned off, and the GFP-Polo dispersed back into the ooplasm.
Date
Source Movie S2 from Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R (2009). "Hypoxia Transiently Sequesters Mps1 and Polo to Collagenase-Sensitive Filaments in Drosophila Prometaphase Oocytes". PLOS ONE. DOI:10.1371/journal.pone.0007544. PMID 19847308. PMC: 2761730.
Author Gilliland W, Vietti D, Schweppe N, Guo F, Johnson T, Hawley R
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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current08:13, 27 May 20136.5 s, 960 × 540 (431 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 480P 252 kbps Completed 06:29, 29 August 2018 6.0 s
Streaming 480p (VP9) 253 kbps Completed 23:41, 21 March 2024 1.0 s
VP9 360P 136 kbps Completed 06:29, 29 August 2018 5.0 s
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VP9 240P 80 kbps Completed 06:29, 29 August 2018 3.0 s
Streaming 240p (VP9) 81 kbps Completed 02:36, 12 January 2024 1.0 s
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Streaming 144p (MJPEG) 960 kbps Completed 04:32, 15 November 2023 1.0 s

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