File:Identification-of-a-Novel-Gammaretrovirus-in-Prostate-Tumors-of-Patients-Homozygous-for-R462Q-ppat.0020025.sv001.ogv
Identification-of-a-Novel-Gammaretrovirus-in-Prostate-Tumors-of-Patients-Homozygous-for-R462Q-ppat.0020025.sv001.ogv (Ogg multiplexed audio/video file, Theora/Vorbis, length 11 s, 364 × 360 pixels, 148 kbps overall, file size: 195 KB)
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[edit]DescriptionIdentification-of-a-Novel-Gammaretrovirus-in-Prostate-Tumors-of-Patients-Homozygous-for-R462Q-ppat.0020025.sv001.ogv |
English: Confocal Optical Image Planes of a Representative XMRV FISH Positive Cell Optical image planes (0.5 μm step-size) of the XMRV FISH positive cell from Figure 1A acquired using a Leica TCS SP2 laser scanning spectral confocal microscope (Leica, Heidelberg, Germany) were reconstructed into a 3D volume set using Volocity 3.5 (Improvision, Lexington, Massachusetts, United States). Using Volocity's movie sequence editor, each volume was rotated along horizontal and vertical axes, adjusting nuclei stained DAPI (blue) channel brightness to visualize underlying XMRV FISH (green) nucleic acid signal. The resulting image frames were exported as a movie sequence. Underlying grid represents glass slide to which tissue was placed for FISH analysis. Each square unit within grid represents 4 μm in height and width. |
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Source | Video S1 from Urisman A, Molinaro R, Fischer N, Plummer S, Casey G, Klein E, Malathi K, Magi-Galluzzi C, Tubbs R, Ganem D, Silverman R, DeRisi J (2006). "Identification of a Novel Gammaretrovirus in Prostate Tumors of Patients Homozygous for R462Q RNASEL Variant". PLOS Pathogens. DOI:10.1371/journal.ppat.0020025. PMID 16609730. PMC: 1434790. | ||
Author | Urisman A, Molinaro R, Fischer N, Plummer S, Casey G, Klein E, Malathi K, Magi-Galluzzi C, Tubbs R, Ganem D, Silverman R, DeRisi J | ||
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current | 08:50, 16 November 2012 | 11 s, 364 × 360 (195 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Confocal Optical Image Planes of a Representative XMRV FISH Positive Cell |
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Author | Urisman A, Molinaro R, Fischer N, Plummer S, Casey G, Klein E, Malathi K, Magi-Galluzzi C, Tubbs R, Ganem D, Silverman R, DeRisi J |
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Optical image planes (0.5 ?m step-size) of the XMRV FISH positive cell from Figure 1A acquired using a Leica TCS SP2 laser scanning spectral confocal microscope (Leica, Heidelberg, Germany) were reconstructed into a 3D volume set using Volocity 3.5 (Improvision, Lexington, Massachusetts, United States). Using Volocity's movie sequence editor, each volume was rotated along horizontal and vertical axes, adjusting nuclei stained DAPI (blue) channel brightness to visualize underlying XMRV FISH (green) nucleic acid signal. The resulting image frames were exported as a movie sequence. Underlying grid represents glass slide to which tissue was placed for FISH analysis. Each square unit within grid represents 4 ?m in height and width. |
Software used | |
Date and time of digitizing | 2006-03 |
Language | English |
- Cancer biology
- Epidemiology - public health
- Videos of infectious diseases and disorders
- Mus musculus
- Adenocarcinoma
- Viral DNA
- Endoribonucleases
- Gammaretrovirus
- Genetic predispositions to diseases
- Viral genome
- In situ hybridization
- Molecular sequence data
- Oligonucleotide array sequence analysis
- Videos of prostatic neoplasms
- Biological tumor markers
- DAPI