File:Imaging-activity-in-astrocytes-and-neurons-with-genetically-encoded-calcium-indicators-following-in-Video3.ogv

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Imaging-activity-in-astrocytes-and-neurons-with-genetically-encoded-calcium-indicators-following-in-Video3.ogv(Ogg Theora video file, length 9.1 s, 300 × 300 pixels, 335 kbps, file size: 371 KB)

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English: Spontaneous activity in a cortical astrocyte expressing Lck-GCaMP6f in an acute brain slice. Slices were obtained from a P34 animal. Astrocyte calcium events appeared more random and much smaller than those seen in organotypic slice cultures. Localized events with large fluorescence changes were frequently observed throughout astrocyte processes with only sparse somatic activity. We did not observe astrocytes with overlapping domains or extended processes, consistent with a non-reactive phenotype. Neuronal events (not shown in this movie) were very sparse with and small in amplitude. The upper right astrocyte in this movie is used as the example cell in Figures 5A,B.
Date
Source Supplementary Movie 3 from Gee J, Gibbons M, Taheri M, Palumbos S, Morris S, Smeal R, Flynn K, Economo M, Cizek C, Capecchi M, Tvrdik P, Wilcox K, White J (2015). "Imaging activity in astrocytes and neurons with genetically encoded calcium indicators following in utero electroporation". Frontiers in Molecular Neuroscience. DOI:10.3389/fnmol.2015.00010. PMC: 4397926.
Author Gee J, Gibbons M, Taheri M, Palumbos S, Morris S, Smeal R, Flynn K, Economo M, Cizek C, Capecchi M, Tvrdik P, Wilcox K, White J
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Date/TimeThumbnailDimensionsUserComment
current15:55, 30 April 20159.1 s, 300 × 300 (371 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 66 kbps Completed 13:10, 29 August 2018 4.0 s
Streaming 240p (VP9) 67 kbps Completed 05:21, 5 December 2023 1.0 s
WebM 360P 79 kbps Completed 14:38, 30 November 2023 1.0 s
Streaming 144p (MJPEG) 267 kbps Completed 20:07, 17 November 2023 1.0 s

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