File:Imaging-activity-in-astrocytes-and-neurons-with-genetically-encoded-calcium-indicators-following-in-Video3.ogv
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Imaging-activity-in-astrocytes-and-neurons-with-genetically-encoded-calcium-indicators-following-in-Video3.ogv (Ogg Theora video file, length 9.1 s, 300 × 300 pixels, 335 kbps, file size: 371 KB)
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DescriptionImaging-activity-in-astrocytes-and-neurons-with-genetically-encoded-calcium-indicators-following-in-Video3.ogv |
English: Spontaneous activity in a cortical astrocyte expressing Lck-GCaMP6f in an acute brain slice. Slices were obtained from a P34 animal. Astrocyte calcium events appeared more random and much smaller than those seen in organotypic slice cultures. Localized events with large fluorescence changes were frequently observed throughout astrocyte processes with only sparse somatic activity. We did not observe astrocytes with overlapping domains or extended processes, consistent with a non-reactive phenotype. Neuronal events (not shown in this movie) were very sparse with and small in amplitude. The upper right astrocyte in this movie is used as the example cell in Figures 5A,B. |
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Source | Supplementary Movie 3 from Gee J, Gibbons M, Taheri M, Palumbos S, Morris S, Smeal R, Flynn K, Economo M, Cizek C, Capecchi M, Tvrdik P, Wilcox K, White J (2015). "Imaging activity in astrocytes and neurons with genetically encoded calcium indicators following in utero electroporation". Frontiers in Molecular Neuroscience. DOI:10.3389/fnmol.2015.00010. PMC: 4397926. | ||
Author | Gee J, Gibbons M, Taheri M, Palumbos S, Morris S, Smeal R, Flynn K, Economo M, Cizek C, Capecchi M, Tvrdik P, Wilcox K, White J | ||
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This file is licensed under the Creative Commons Attribution 4.0 International license.
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current | 15:55, 30 April 2015 | 9.1 s, 300 × 300 (371 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Gee J, Gibbons M, Taheri M, Palumbos S, Morris S, Smeal R, Flynn K, Economo M, Cizek C, Capecchi M, Tvrdik P, Wilcox K, White J |
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Usage terms | http://creativecommons.org/licenses/by/4.0/ |
Image title | Spontaneous activity in a cortical astrocyte expressing Lck-GCaMP6f in an acute brain slice. Slices were obtained from a P34 animal. Astrocyte calcium events appeared more random and much smaller than those seen in organotypic slice cultures. Localized events with large fluorescence changes were frequently observed throughout astrocyte processes with only sparse somatic activity. We did not observe astrocytes with overlapping domains or extended processes, consistent with a non-reactive phenotype. Neuronal events (not shown in this movie) were very sparse with and small in amplitude. The upper right astrocyte in this movie is used as the example cell in Figures 5A,B. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2015-04-15 |