File:Live-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S2.ogv
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DescriptionLive-cell-imaging-techniques-to-study-T-cell-trafficking-across-the-blood-brain-barrier-in-vitro-2045-8118-10-7-S2.ogv |
English: Movie 2. Shear-resistant arrest, polarization, crawling and diapedesis of CD4+ T cells on and across TNF-α stimulated wt pMBMECs under flow (high magnification). The experimental setup was identical to that described in Movie 1. Images were taken with a 40x objective (Objective LD “Plan-Neofluar” 40x/0,6 Korr Ph2 M27) under differential interference contrast illumination at 3 images per min; recording time 14.5 min. Movie at 8 images per sec; field of view 215 μm x 162 μm. |
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Source | Coisne C, Lyck R, Engelhardt B (2013). "Live cell imaging techniques to study T cell trafficking across the blood-brain barrier in vitro and in vivo". Fluids and Barriers of the CNS. DOI:10.1186/2045-8118-10-7. PMID 23336847. PMC: 3560242. | ||
Author | Coisne C, Lyck R, Engelhardt B | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 21:31, 9 February 2013 | 5.5 s, 1,384 × 1,040 (5.65 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional file 2 |
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Author | Coisne C, Lyck R, Engelhardt B |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Movie 2. Shear-resistant arrest, polarization, crawling and diapedesis of CD4+ T cells on and across TNF-? stimulated wt pMBMECs under flow (high magnification). The experimental setup was identical to that described in Movie 1. Images were taken with a 40x objective (Objective LD ?Plan-Neofluar? 40x/0,6 Korr Ph2 M27) under differential interference contrast illumination at 3 images per min; recording time 14.5?min. Movie at 8 images per sec; field of view 215??m x 162??m. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |