File:Multicellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s011.ogv
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[edit]DescriptionMulticellular-Bacteria-Deploy-the-Type-VI-Secretion-System-to-Preemptively-Strike-Neighboring-Cells-ppat.1003608.s011.ogv |
English: Live-cell fluorescence microscopy capturing the infiltration of P. mirabilis HI4320 within the opposing 9C1 mutant swarm following merging of the two populations. Strain HI4320 expressing VipA::sfGFP under control of pBAD is observed infiltrating deeply into the opposing 9C1 swarm labeled with dsRED. Images are taken in real-time approximately 3 mm beyond the merge point within the 9C1 swarm. Only individual wild-type HI4320 swarmer cells are observed in the 9C1 swarm and appear to maintain the ability to rapidly migrate within the opposing swarm. Swarms were visualized on swarm agar containing 10 mM L-arabinose to induce expression of the VipA::sfGFP translational fusion. Single channel fluorescence, brightfield, and merged images are presented. |
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Source | Movie S2 from Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H (2013). "Multicellular Bacteria Deploy the Type VI Secretion System to Preemptively Strike Neighboring Cells". PLOS Pathogens. DOI:10.1371/journal.ppat.1003608. PMC: 3764213. | ||
Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H | ||
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current | 20:29, 14 September 2013 | 5.0 s, 1,392 × 1,040 (924 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Alteri C, Himpsl S, Pickens S, Lindner J, Zora J, Miller J, Arno P, Straight S, Mobley H |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Live-cell fluorescence microscopy capturing the infiltration of P. mirabilis HI4320 within the opposing 9C1 mutant swarm following merging of the two populations. Strain HI4320 expressing VipA::sfGFP under control of pBAD is observed infiltrating deeply into the opposing 9C1 swarm labeled with dsRED. Images are taken in real-time approximately 3 mm beyond the merge point within the 9C1 swarm. Only individual wild-type HI4320 swarmer cells are observed in the 9C1 swarm and appear to maintain the ability to rapidly migrate within the opposing swarm. Swarms were visualized on swarm agar containing 10 mM L-arabinose to induce expression of the VipA::sfGFP translational fusion. Single channel fluorescence, brightfield, and merged images are presented. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-09 |