File:Node Cilia Are Posteriorly Tilted and Positioned primitive node.png
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Figure 3. Node Cilia Are Posteriorly Tilted and Positioned (A) Scanning electron micrograph of the wild-type node. Note that cilia emanate from the posterior part of the cells. The view angle is about 30° with respect to the horizontal line. (B, C) Scanning electron micrograph of the iv/iv node. (C) is a high-magnification picture of the region in (B) indicated by an arrow. (D) Deduced tilt of iv/iv node cilia after stereography from multiple-tilt scanning electron micrograph images. Yellow lines indicate cilia, red dots their root positions, and a blue square a plane best-fit to the node surface. When we calculated the tilt of the cilia, we separated the tilt into A-P (anterior–posterior) and L-R components. The average tilt was 26.6° in A-P axis (toward the posterior) and 0.06° in L-R axis (towards the right). (E) Immunofluorecence image of node cells shown as projection of 3D confocal data stack. Basal bodies and cell boundaries are shown by immunofluorescence against γ-tubulin (red) and ZO-1 (green), respectively. (F) 3D reconstruction of (E) viewed from ventral side (top) and right side (bottom), showing posterior bias of basal body positions. White lines divide the cells into the anterior and the posterior halves. Basal bodies located in the anterior and the posterior are shown in yellow and red, respectively. (G) Speculative interpretation of posterior bias of basal bodies in orientation and position of the node cilia. Because the node cells are somewhat rounded, if basal bodies were located at the posterior part of these cells, it would result in posteriorly tilted cilia even though the basal bodies remain perpendicular to the plasma membrane |
| Date | |
| Source | https://doi.org/10.1371/journal.pbio.0030268 (2005) De Novo Formation of Left–Right Asymmetry by Posterior Tilt of Nodal Cilia. PLoS Biol 3(8): e268. |
| Author | Nonaka S, Yoshiba S, Watanabe D, Ikeuchi S, Goto T, Marshall WF, et al. |
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Copyright: © 2005 Nonaka et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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| current | 20:30, 4 May 2024 | | 2,020 × 3,157 (4.01 MB) | Rasbak (talk | contribs) | {{Information |description=Figure 3. Node Cilia Are Posteriorly Tilted and Positioned (A) Scanning electron micrograph of the wild-type node. Note that cilia emanate from the posterior part of the cells. The view angle is about 30° with respect to the horizontal line. (B, C) Scanning electron micrograph of the iv/iv node. (C) is a high-magnification picture of the region in (B) indicated by an arrow. (D) Deduced tilt of iv/iv node cilia after stereography from multiple-tilt scanning electron... |
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