File:Protein-Complexes-and-Proteolytic-Activation-of-the-Cell-Wall-Hydrolase-RipA-Regulate-Septal-ppat.1003197.s010.ogv

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Original file ‎(Ogg Theora video file, length 15 s, 1,024 × 1,024 pixels, 8.49 Mbps, file size: 14.93 MB)

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English: RipASm overexpressed cells lyse. M. smegmatis carrying a plasmid in which wildtype RipASm was fused to GFP was grown on an agar pad with the inducer aTc, in order to overexpress wild-type RipA. Micrographs of the GFP signal were captured every 10 minutes by fluorescence microscopy and a time-lapse movie was compiled from the individual timepoints. Lysis of RipA expressing cells can be identified by loss of GFP between time points.
Date
Source Video S1 from Chao M, Kieser K, Minami S, Mavrici D, Aldridge B, Fortune S, Alber T, Rubin E (2013). "Protein Complexes and Proteolytic Activation of the Cell Wall Hydrolase RipA Regulate Septal Resolution in Mycobacteria". PLOS Pathogens. DOI:10.1371/journal.ppat.1003197. PMID 23468634. PMC: 3585148.
Author Chao M, Kieser K, Minami S, Mavrici D, Aldridge B, Fortune S, Alber T, Rubin E
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This file is licensed under the Creative Commons Attribution 2.5 Generic license.
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This file was published in a Public Library of Science journal. Their website states that the content of all PLOS journals is published under the Creative Commons Attribution 4.0 license (or its previous version depending on the publication date), unless indicated otherwise.
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Date/TimeThumbnailDimensionsUserComment
current19:42, 8 March 201315 s, 1,024 × 1,024 (14.93 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 957 kbps Completed 07:17, 16 October 2018 12 s
Streaming 720p (VP9) Not ready Unknown status
VP9 480P 297 kbps Completed 07:17, 16 October 2018 6.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 97 kbps Completed 07:17, 16 October 2018 4.0 s
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VP9 240P 48 kbps Completed 07:17, 16 October 2018 3.0 s
Streaming 240p (VP9) 48 kbps Completed 23:53, 15 December 2023 1.0 s
WebM 360P 517 kbps Completed 19:44, 8 March 2013 9.0 s
Streaming 144p (MJPEG) 112 kbps Completed 16:11, 20 November 2023 1.0 s

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