File:Quantitative-3-Dimensional-Imaging-of-Murine-Neointimal-and-Atherosclerotic-Lesions-by-Optical-pone.0016906.s003.ogv

English: Volume rendering of a tomographically reconstructed, OPT-imaged, atherosclerotic aortic arch. After tomographic reconstruction, cross-sectional OPT images (excitation 425 nm with 40 nm band-pass; emission 475 nm long pass) can be volume rendered to visualise vascular structure and lesion formation in 3-dimensions. In atherosclerotic aortic arches, presented in this way (a), lesion formation can be observed with the predicated anatomical distribution (brachiocephalic artery, roots of the right carotid and subclavian arteries and lesser curvature of the arch). Regions within cross-sections corresponding to lesion can be segmented, separately rendered, and superimposed on original data to further highlight distribution and extent of lesion formation (b; lesion displayed in red). This process is analogous to that required to quantify lesion volume.