File:RNA-interference-in-marine-and-freshwater-sponges-actin-knockdown-in-Tethya-wilhelma-and-Ephydatia-1472-6750-11-67-S2.ogv

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RNA-interference-in-marine-and-freshwater-sponges-actin-knockdown-in-Tethya-wilhelma-and-Ephydatia-1472-6750-11-67-S2.ogv(Ogg Theora video file, length 44 s, 286 × 192 pixels, 43 kbps, file size: 229 KB)

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English: Additional File 2 Viability check of RNAi-treated T. wilhelma specimens. Time-lapse imaging and image analysis was used to address contractility as a viability marker. Time-lapse image movie of the specimen representing the knockdown value at 201 hours in the long-term study (Figure 3C and Figure 4). The specimen is dislocated at the time of RNAi feeding due to liquid handling (time: 12:24 hh:mm; see Figure 4; culture dish had to be temporarily removed from the imaging station for feeding and was moved back afterwards).
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Source Rivera A, Hammel J, Haen K, Danka E, Cieniewicz B, Winters I, Posfai D, Wörheide G, Lavrov D, Knight S, Hill M, Hill A, Nickel M (2011). "RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleri by ingested dsRNA expressing bacteria". BMC Biotechnology. DOI:10.1186/1472-6750-11-67. PMID 21679422. PMC: 3146823.
Author Rivera A, Hammel J, Haen K, Danka E, Cieniewicz B, Winters I, Posfai D, Wörheide G, Lavrov D, Knight S, Hill M, Hill A, Nickel M
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Date/TimeThumbnailDimensionsUserComment
current19:42, 6 December 201244 s, 286 × 192 (229 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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