File:RNA-interference-in-marine-and-freshwater-sponges-actin-knockdown-in-Tethya-wilhelma-and-Ephydatia-1472-6750-11-67-S2.ogv
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RNA-interference-in-marine-and-freshwater-sponges-actin-knockdown-in-Tethya-wilhelma-and-Ephydatia-1472-6750-11-67-S2.ogv (Ogg Theora video file, length 44 s, 286 × 192 pixels, 43 kbps, file size: 229 KB)
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DescriptionRNA-interference-in-marine-and-freshwater-sponges-actin-knockdown-in-Tethya-wilhelma-and-Ephydatia-1472-6750-11-67-S2.ogv |
English: Additional File 2 Viability check of RNAi-treated T. wilhelma specimens. Time-lapse imaging and image analysis was used to address contractility as a viability marker. Time-lapse image movie of the specimen representing the knockdown value at 201 hours in the long-term study (Figure 3C and Figure 4). The specimen is dislocated at the time of RNAi feeding due to liquid handling (time: 12:24 hh:mm; see Figure 4; culture dish had to be temporarily removed from the imaging station for feeding and was moved back afterwards). |
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Date | |||
Source | Rivera A, Hammel J, Haen K, Danka E, Cieniewicz B, Winters I, Posfai D, Wörheide G, Lavrov D, Knight S, Hill M, Hill A, Nickel M (2011). "RNA interference in marine and freshwater sponges: actin knockdown in Tethya wilhelma and Ephydatia muelleri by ingested dsRNA expressing bacteria". BMC Biotechnology. DOI:10.1186/1472-6750-11-67. PMID 21679422. PMC: 3146823. | ||
Author | Rivera A, Hammel J, Haen K, Danka E, Cieniewicz B, Winters I, Posfai D, Wörheide G, Lavrov D, Knight S, Hill M, Hill A, Nickel M | ||
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This file is licensed under the Creative Commons Attribution 2.0 Generic license.
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current | 19:42, 6 December 2012 | 44 s, 286 × 192 (229 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Short title | Additional File 2 |
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Author | |
Usage terms | http://creativecommons.org/licenses/by/2.0/ |
Image title | Viability check of RNAi-treated T. wilhelma specimens. Time-lapse imaging and image analysis was used to address contractility as a viability marker. Time-lapse image movie of the specimen representing the knockdown value at 201 hours in the long-term study (Figure 3C and Figure 4). The specimen is dislocated at the time of RNAi feeding due to liquid handling (time: 12:24 hh:mm; see Figure 4; culture dish had to be temporarily removed from the imaging station for feeding and was moved back afterwards). |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2011 |