File:Regulation-of-T-Cell-Motility-In-Vitro-and-In-Vivo-by-LPA-and-LPA2-pone.0101655.s001.ogv

English: Lpa2-deficiency results in early defects in T cell migration at the HEV (Supplemental to Figure 5). Representative two-photon microscopic images obtained from the popliteal lymph node of wild-type mice. Wild-type and lpa2−/− CD4+ T cells were labeled with CFSE and CMTMR, respectively and (5–10)×106 cells mixed in a 1∶1 ratio together with Texas Red Dextran were injected into wild-type recipients through the orbital sinus. MP-IVM was performed on a microsurgically exposed popliteal lymph node to visualize T cell motility during extravasation at the HEV. The video shows the first 31 minutes of imaging, which is representative of migration over the course of an hour.