File:Rgs13-Constrains-Early-B-Cell-Responses-and-Limits-Germinal-Center-Sizes-pone.0060139.s005.ogv
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Rgs13-Constrains-Early-B-Cell-Responses-and-Limits-Germinal-Center-Sizes-pone.0060139.s005.ogv (Ogg Theora video file, length 2.5 s, 700 × 526 pixels, 7.15 Mbps, file size: 2.13 MB)
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[edit]DescriptionRgs13-Constrains-Early-B-Cell-Responses-and-Limits-Germinal-Center-Sizes-pone.0060139.s005.ogv |
English: Intravital microscopy of the inguinal lymph node from a Rgs13GFP KI mouse 2D post immunization identifies an expanding population of GFP positive cells located at the edge of B cell follicle. Shown is the distribution and behavior of GFP expressing KI cells in the inguinal lymph node of a KI mouse. Images were taken at D2 post immunization via intra-peritoneal injection of sRBCs. An image sequence of an 80 μm z- projection was acquired with 20× lens over 30 minutes. From shallow (top left) to deep (low right), each panel is from a 80 μm volume image stack at 10 μm intervals moving from the LN surface deeper into the lymph node cortex. The capsule of the lymph nodes was visualized by second harmonic signal (blue) from collagen. Adoptively transferred B cells labeled with eFluor® 670 are shown in white. The follicular dendritic cell (FDC) network (red) was visualized by means of subcutaneous injection of anti-CD21/35 PE conjugated antibody (3 μg) prior to imaging. GFP signals are shown in the green channel. White lines indicate edge of the B-cell follicle based on distribution of WT B cells adoptively transferred 1D prior to imaging. The scale bar is 150 μm. Time counter is h:min:sec. |
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Source | Video S2 from Hwang I, Hwang K, Park C, Harrison K, Kehrl J (2013). "Rgs13 Constrains Early B Cell Responses and Limits Germinal Center Sizes". PLOS ONE. DOI:10.1371/journal.pone.0060139. PMID 23533672. PMC: 3606317. | ||
Author | Hwang I, Hwang K, Park C, Harrison K, Kehrl J | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 08:42, 3 June 2013 | 2.5 s, 700 × 526 (2.13 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Hwang I, Hwang K, Park C, Harrison K, Kehrl J |
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Usage terms | http://creativecommons.org/publicdomain/zero/1.0/ |
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Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013 |