File:Rgs13-Constrains-Early-B-Cell-Responses-and-Limits-Germinal-Center-Sizes-pone.0060139.s006.ogv

English: Intravital microscopy of the inguinal lymph node from a Rgs13GFP KI mouse 8D post immunization identifies a large population of GFP positive cells residing in a germinal center located in the LN follicle. Shown is the distribution and behavior of GFP expressing KI cells in the LN follicle of the inguinal lymph node of a KI mouse. Images were taken D8 post immunization via intra-peritoneal injection of sRBCs. An image sequence of an 80 μm z- projection was acquired with 25x lens over 30 minutes. From shallow (top left) to deep (low right), each panel is from the 80 μm volume image stack at 10 μm intervals moving from the LN surface deeper into the lymph node cortex. The capsule of the lymph nodes was visualized by second harmonic signal (blue) from collagen. Adoptively transferred B cells labeled with CMF2HC are shown in blue. The follicular dendritic cell (FDC) network (red) was visualized by means of subcutaneous injection of anti-CD21/35 PE conjugated antibody (3 μg) prior to imaging. GFP signals are in the green channel. White lines indicate edge of the B-cell follicle, and were drawn based on distribution of WT B cells adoptively transferred 1D prior to imaging. The scale bar is 200 μm. Time counter is h:min:sec.