File:Sorting-Nexin-6-Enhances-Lamin-A-Synthesis-and-Incorporation-into-the-Nuclear-Envelope-pone.0115571.s005.ogv

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English: Localization of lamin A at the endoplasmic reticulum upon the overexpression of SNX6. (A) Imaris 3D reconstruction of in vivo time-lapse confocal microscopy analysis of U2OS cells cotransfected with GFP-lamin A, HA-SNX6 (to promote extranuclear lamin A accumulation) and RFP-SEC61 (ER label). ER (red) and GFP-lamin A (green) and colocalization of both (yellow). U2OS cells cotransfected with RFP-Sec-61, GFP-Lamin A and HA-SNX6 were examined under a TCS SP5 confocal laser scanning unit attached to an inverted epifluorescence microscope (DMI6000) fitted with an HCX PL APO 63/NA 1.40-0.60 oil objective. Cells were maintained in DMEM (containing 10%FBS and 20 mM Hepes) in 35 mm dishes (MatTek) at 37°C in a 5% CO2 atmosphere.
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Source S1 Video from González-Granado J, Navarro-Puche A, Molina-Sanchez P, Blanco-Berrocal M, Viana R, de Mora J, Andrés V (2014). "Sorting Nexin 6 Enhances Lamin A Synthesis and Incorporation into the Nuclear Envelope". PLOS ONE. DOI:10.1371/journal.pone.0115571. PMID 25535984. PMC: 4275242.
Author González-Granado J, Navarro-Puche A, Molina-Sanchez P, Blanco-Berrocal M, Viana R, de Mora J, Andrés V
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current16:22, 6 April 20154.5 s, 1,440 × 768 (352 KB)McZusatz (talk | contribs)c:User:Rillke/bigChunkedUpload.js: converted from src
22:17, 5 January 2015213,502 d 21 h 3 min 54 s, 1,440 × 755 (469 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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