File:Strong-inter-population-cooperation-leads-to-partner-intermixing-in-microbial-communities-elife00230v001.ogv
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[edit]DescriptionStrong-inter-population-cooperation-leads-to-partner-intermixing-in-microbial-communities-elife00230v001.ogv |
English: Yeast cells bud to the sides when there is available space and bud upward when sufficiently confined (corresponding to Figure 1—figure supplement 1A). To infer the process of cell rearrangement in three-dimensional communities, we monitored how single YFP-fluorescent yeast cells grew into microcolonies on top of solid agarose. Initially, dividing cells pushed other cells to the side such that all cells remained in the same plane. When a cell was sufficiently confined from the sides by other cells (approximately within a 5-cell radius), it could no longer bud to the side and instead budded upward, as indicated by higher intensities in the fluorescence images. Continued growth of microcolony forced more cells in the middle to send their progeny to upper layers, while cells close to the edge could still push other cells to the side and remain on the agarose surface. All images are taken with the same exposure time. Scale bar is 20 μm.
DOI: https://dx.doi.org/10.7554/eLife.00230.008 |
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Source | Video 1. from Momeni B, Brileya K, Fields M, Shou W (2013). "Strong inter-population cooperation leads to partner intermixing in microbial communities". eLife. DOI:10.7554/eLife.00230. | ||
Author | Momeni B, Brileya K, Fields M, Shou W | ||
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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current | 12:15, 29 January 2013 | 1.9 s, 1,344 × 1,008 (179 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Momeni B, Brileya K, Fields M, Shou W |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Yeast cells bud to the sides when there is available space and bud upward when sufficiently confined (corresponding to Figure 1?figure supplement 1A). To infer the process of cell rearrangement in three-dimensional communities, we monitored how single YFP-fluorescent yeast cells grew into microcolonies on top of solid agarose. Initially, dividing cells pushed other cells to the side such that all cells remained in the same plane. When a cell was sufficiently confined from the sides by other cells (approximately within a 5-cell radius), it could no longer bud to the side and instead budded upward, as indicated by higher intensities in the fluorescence images. Continued growth of microcolony forced more cells in the middle to send their progeny to upper layers, while cells close to the edge could still push other cells to the side and remain on the agarose surface. All images are taken with the same exposure time. Scale bar is 20 ?m.
DOI: http://dx.doi.org/10.7554/eLife.00230.008 |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2013-01-22 |