File:Strong-inter-population-cooperation-leads-to-partner-intermixing-in-microbial-communities-elife00230v001.ogv

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English: Yeast cells bud to the sides when there is available space and bud upward when sufficiently confined (corresponding to Figure 1—figure supplement 1A). To infer the process of cell rearrangement in three-dimensional communities, we monitored how single YFP-fluorescent yeast cells grew into microcolonies on top of solid agarose. Initially, dividing cells pushed other cells to the side such that all cells remained in the same plane. When a cell was sufficiently confined from the sides by other cells (approximately within a 5-cell radius), it could no longer bud to the side and instead budded upward, as indicated by higher intensities in the fluorescence images. Continued growth of microcolony forced more cells in the middle to send their progeny to upper layers, while cells close to the edge could still push other cells to the side and remain on the agarose surface. All images are taken with the same exposure time. Scale bar is 20 μm.

DOI:

https://dx.doi.org/10.7554/eLife.00230.008
Date
Source Video 1. from Momeni B, Brileya K, Fields M, Shou W (2013). "Strong inter-population cooperation leads to partner intermixing in microbial communities". eLife. DOI:10.7554/eLife.00230.
Author Momeni B, Brileya K, Fields M, Shou W
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This file is licensed under the Creative Commons Attribution 3.0 Unported license.
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current12:15, 29 January 20131.9 s, 1,344 × 1,008 (179 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 720P 344 kbps Completed 19:33, 20 October 2018 3.0 s
Streaming 720p (VP9) Not ready Unknown status
VP9 480P 217 kbps Completed 19:33, 20 October 2018 2.0 s
Streaming 480p (VP9) Not ready Unknown status
VP9 360P 126 kbps Completed 19:33, 20 October 2018 1.0 s
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VP9 240P 65 kbps Completed 19:33, 20 October 2018 2.0 s
Streaming 240p (VP9) 67 kbps Completed 23:54, 16 December 2023 0.0 s
WebM 360P 50 kbps Completed 12:17, 29 January 2013 1.0 s
Streaming 144p (MJPEG) 817 kbps Completed 13:59, 19 November 2023 1.0 s

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