File:The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S5.ogv

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The-Amyloid-Precursor-Protein-is-rapidly-transported-from-the-Golgi-apparatus-to-the-lysosome-and-s13041-014-0054-1-S5.ogv ‎(Ogg Theora video file, length 12 s, 352 × 288 pixels, 87 kbps, file size: 122 KB)

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English: Additional file 5: Video S2/Figure 1. APP paGFP is accurately photoactivated in the Golgi apparatus. SN56 cells were transiently transfected with GalT-CFP to identify the Golgi apparatus, LAMP1-mRFP to identify lysosomes, and βAPP-paGFP and were treated with Nocodozole to block exit from the Golgi. Irradiation targets (circles) were drawn over the Golgi apparatus and the were irradiated with 405 nm laser light, alternating with imaging for 15 minutes (indicated by the green word ‘photoactivating’ on the images. Cells were then followed in a ‘chase period’ imaging every 30 seconds for the time indicated. Photoactivated βAPP-paGFP can be seen accumulating in the Golgi.
Date
Source Video file from Tam J, Seah C, Pasternak S (2014). "The Amyloid Precursor Protein is rapidly transported from the Golgi apparatus to the lysosome and where it is processed into beta-amyloid". Molecular Brain. DOI:10.1186/s13041-014-0054-1. PMID 25085554. PMC: 4237969.
Author Tam J, Seah C, Pasternak S
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current12:05, 22 November 201412 s, 352 × 288 (122 KB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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Format Bitrate Download Status Encode time
VP9 240P 38 kbps Completed 17:13, 21 October 2018 3.0 s
Streaming 240p (VP9) 38 kbps Completed 15:38, 17 December 2023 1.0 s
WebM 360P 57 kbps Completed 15:15, 2 December 2023 0.0 s
Streaming 144p (MJPEG) 315 kbps Completed 09:16, 9 November 2023 0.0 s

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