File:The-Diverse-and-Dynamic-Nature-of-Leishmania-Parasitophorous-Vacuoles-Studied-by-Multidimensional-pntd.0001518.s006.ogv
From Wikimedia Commons, the free media repository
Jump to navigation
Jump to search
No higher resolution available.
The-Diverse-and-Dynamic-Nature-of-Leishmania-Parasitophorous-Vacuoles-Studied-by-Multidimensional-pntd.0001518.s006.ogv (Ogg Theora video file, length 56 s, 344 × 364 pixels, 466 kbps, file size: 3.1 MB)
File information
Structured data
Captions
Summary
[edit]DescriptionThe-Diverse-and-Dynamic-Nature-of-Leishmania-Parasitophorous-Vacuoles-Studied-by-Multidimensional-pntd.0001518.s006.ogv |
English: Fission of L. major PVs. (A) Fission inferred by differences in Lysotracker RFIs surrounding dividing parasites. Multidimensional imaging of dividing L. major-DsRed2 amastigotes (red) hosted by a macrophage loaded with Lysotracker (green). Isosurfaces were attributed to replicating amastigotes, allowing the measurement of Lysotracker RFI surrounding parasites. The amastigotes remain next to each other after replication and the vacuolar interface between them is observed at the initial time points. An increase in the Lysotracker signal was detected in one dividing amastigote, whereas the other remained in a PV with a low Lysotracker signal. By the end of the recordings, the amastigotes were surrounded by Lysotracker at low intensities, and no Lysotracker-positive vacuolar interface was observed between them. Image acquisition started after 48 h of infection, and the time of acquisition is shown as hh:mm. (B) L. major PV fission observed in infected RAW 264.7 macrophages expressing LAMP1 and Rab7 tagged with GFP. A double-occupancy PV precedes the formation of individualized L. major PVs, between time points 10:40 and 12:31. Fission is completed after the incorporation of phagolysosomal-membrane in the interface between dividing parasites (time point 12:31). Image acquisition started after 3 h of infection, and the time of acquisition is shown as dhh:mm. |
||
Date | |||
Source | Video S6 from Real F, Mortara R (2012). "The Diverse and Dynamic Nature of Leishmania Parasitophorous Vacuoles Studied by Multidimensional Imaging". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0001518. PMID 22348167. PMC: 3279510. | ||
Author | Real F, Mortara R | ||
Permission (Reusing this file) |
|
||
Provenance InfoField |
|
File history
Click on a date/time to view the file as it appeared at that time.
Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 23:11, 5 June 2013 | 56 s, 344 × 364 (3.1 MB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
You cannot overwrite this file.
File usage on Commons
There are no pages that use this file.
Transcode status
Update transcode statusMetadata
This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.
Author | Real F, Mortara R |
---|---|
Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | Fission of L. major PVs. (A) Fission inferred by differences in Lysotracker RFIs surrounding dividing parasites. Multidimensional imaging of dividing L. major-DsRed2 amastigotes (red) hosted by a macrophage loaded with Lysotracker (green). Isosurfaces were attributed to replicating amastigotes, allowing the measurement of Lysotracker RFI surrounding parasites. The amastigotes remain next to each other after replication and the vacuolar interface between them is observed at the initial time points. An increase in the Lysotracker signal was detected in one dividing amastigote, whereas the other remained in a PV with a low Lysotracker signal. By the end of the recordings, the amastigotes were surrounded by Lysotracker at low intensities, and no Lysotracker-positive vacuolar interface was observed between them. Image acquisition started after 48 h of infection, and the time of acquisition is shown as hh:mm. (B) L. major PV fission observed in infected RAW 264.7 macrophages expressing LAMP1 and Rab7 tagged with GFP. A double-occupancy PV precedes the formation of individualized L. major PVs, between time points 10:40 and 12:31. Fission is completed after the incorporation of phagolysosomal-membrane in the interface between dividing parasites (time point 12:31). Image acquisition started after 3 h of infection, and the time of acquisition is shown as dhh:mm. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2012-02 |