File:The-Herpes-Virus-Fc-Receptor-gE-gI-Mediates-Antibody-Bipolar-Bridging-to-Clear-Viral-Antigens-from-ppat.1003961.s005.ogv

English: 4-D movie of ABB-dependent trafficking of gD and anti-gDhFc to lysosomes (corresponds to Figure 3A). Live cell imaging of HeLa cells expressing gE-gI and gD-Dendra2 (green) incubated with EGF (red) and anti-gDhFc (blue). Regions of EGF-gD colocalization appear yellow; regions of gD-IgG colocalization appear cyan, regions of EGF-IgG colocalization appear magenta, and regions of triple colocalization appear white. 4-D multi-channel confocal imaging was performed using a 63× oil objective lens (αPlan-APOCHROMAT 1.45 Oil DIC) on a LSM510 microscope (Zeiss) and an electron-multiplying charge-coupled device (CCD) camera (Hamamatsu Photonics), controlled by the ZEN 2009 software (Zeiss). Z-stacks (at 1 µm section thickness and up to 16 µm total depth) were captured approximately every 3 min for ∼90 min. The video was recorded at a time resolution of approximately 5 seconds per frame and presented at 10 frames per second. The equatorial planes for z-stack sections are shown on this video.