File:The-N-Myc-Down-Regulated-Gene1-(NDRG1)-Is-a-Rab4a-Effector-Involved-in-Vesicular-Recycling-of-E-pone.0000844.s006.ogv
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The-N-Myc-Down-Regulated-Gene1-(NDRG1)-Is-a-Rab4a-Effector-Involved-in-Vesicular-Recycling-of-E-pone.0000844.s006.ogv (Ogg Theora video file, length 8.4 s, 216 × 335 pixels, 306 kbps, file size: 315 KB)
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[edit]DescriptionThe-N-Myc-Down-Regulated-Gene1-(NDRG1)-Is-a-Rab4a-Effector-Involved-in-Vesicular-Recycling-of-E-pone.0000844.s006.ogv |
English: NDRG1 interacts with recycling E-cadherin. NDRG1DsRed2-HEK293 cells were transfected with pCMVE-cadherinEGFP construct. Twenty four hours post transfection calcium was chelated with EDTA (2.5mM) and cells were plated in calcium-supplemented media before being imaged. Cells were imaged using the Utraview LCI (Perkin Elmer) live cell confocal microscope. Images were grabbed at the rate of 1image/sec and processed using ImageJ software before being made into a movie. NDRG1 vesicles near the perinuclear space and close to the surface of the membrane localizes with E-cadherin as it is trafficked back to the cell surface. |
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Source | Movie S4 from Kachhap S, Faith D, Qian D, Shabbeer S, Galloway N, Pili R, Denmeade S, DeMarzo A, Carducci M (2007). "The N-Myc Down Regulated Gene1 (NDRG1) Is a Rab4a Effector Involved in Vesicular Recycling of E-Cadherin". PLOS ONE. DOI:10.1371/journal.pone.0000844. PMID 17786215. PMC: 1952073. | ||
Author | Kachhap S, Faith D, Qian D, Shabbeer S, Galloway N, Pili R, Denmeade S, DeMarzo A, Carducci M | ||
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Date/Time | Thumbnail | Dimensions | User | Comment | |
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current | 23:38, 14 November 2012 | 8.4 s, 216 × 335 (315 KB) | Open Access Media Importer Bot (talk | contribs) | Automatically uploaded media file from Open Access source. Please report problems or suggestions here. |
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Author | Kachhap S, Faith D, Qian D, Shabbeer S, Galloway N, Pili R, Denmeade S, DeMarzo A, Carducci M |
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Usage terms | http://creativecommons.org/licenses/by/3.0/ |
Image title | NDRG1 interacts with recycling E-cadherin. NDRG1DsRed2-HEK293 cells were transfected with pCMVE-cadherinEGFP construct. Twenty four hours post transfection calcium was chelated with EDTA (2.5mM) and cells were plated in calcium-supplemented media before being imaged. Cells were imaged using the Utraview LCI (Perkin Elmer) live cell confocal microscope. Images were grabbed at the rate of 1image/sec and processed using ImageJ software before being made into a movie. NDRG1 vesicles near the perinuclear space and close to the surface of the membrane localizes with E-cadherin as it is trafficked back to the cell surface. |
Software used | Xiph.Org libtheora 1.1 20090822 (Thusnelda) |
Date and time of digitizing | 2007 |