File:The-study-of-muscle-remodeling-in-Drosophila-metamorphosis-using-in-vivo-microscopy-and-bioimage-1471-2105-13-S17-S14-S1.ogv

English: Temporal comparisons of time-lapse images facilitate the discovery of phenotypic abnormalities resulting from genetic perturbations. The movie shows metamorphosis of two genotypes at 30 minute intervals, a control specimen (left panel) expressing Grasp65-GFP (green) and Histone-mKO (red) and specimen expressing EAST(1-1902)-GFP (right panel), tau-GFP (green) and Histone-mKO (red). The time-lapse starts during the prepupal stage (-6 hours) and last until late pupal stage (+88 hours). The transition from the prepupal to pupal stage begins at zero hours. Note that EAST(1-1902)-GFP overexpression lead to suppression of muscle histolysis in the first 25 hours of the pupal stage and abnormalities of muscle morphology from +38 hours onwards. See Figure 3 for more details.