File:Tonic-Shock-Induces-Detachment-of--Giardia-lamblia-pntd.0000169.s001.ogv

From Wikimedia Commons, the free media repository
Jump to navigation Jump to search

Tonic-Shock-Induces-Detachment-of--Giardia-lamblia-pntd.0000169.s001.ogv ‎(Ogg Theora video file, length 41 s, 450 × 450 pixels, 146 kbps, file size: 722 KB)

Captions

Captions

Add a one-line explanation of what this file represents

Summary[edit]

Description
English: Detachment of fluorescently labeled Giardia from C2 BBe -1 monolayer under periodic tonicity swings of +/−150 mOsm/kg. 
          Movie plays at 50X real time. Unattached cells were flushed out with growth medium (tonicity = 320 mOsm/kg) prior to the movie. The first few frames of the movie show the remainder of this growth medium flowing through the chamber. Cells are then exposed to hypertonic medium (470 mOsm/kg) for 8 minutes (3 minutes of flow at 0.3 mL/min, then 5 minutes static exposure). Cells do not detach during this time, but they become adjusted to the higher tonicity and are primed for detachment during the next 8 minutes of exposure to hypotonic medium (150 mOsm/kg). Media alternate between hyper- and hypotonic to the end of the movie, and additional cells detach with every change.
(8.82 MB MOV)
Date
Source Video S1. from Hansen W, Fletcher D, Lohia A. "Tonic Shock Induces Detachment of Giardia lamblia". PLOS Neglected Tropical Diseases. DOI:10.1371/journal.pntd.0000169. PMID 18270543. PMC: 2238710.
Author Hansen W, Fletcher D, Lohia A
Permission
(Reusing this file)
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 3.0 Unported license.
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
Copyright owner: Hansen, Fletcher.
Provenance
InfoField
This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
WikiProject Open Access
WikiProject Open Access

File history

Click on a date/time to view the file as it appeared at that time.

Date/TimeThumbnailDimensionsUserComment
current22:58, 30 August 201241 s, 450 × 450 (722 KB)Open Access Media Importer Bot (talk | contribs)Uploaded with the Open Access Media Importer. (test edit) botrequest

You cannot overwrite this file.

There are no pages that use this file.

Transcode status

Update transcode status
Format Bitrate Download Status Encode time
VP9 360P 36 kbps Completed 18:46, 23 October 2018 8.0 s
Streaming 360p (VP9) Not ready Unknown status
VP9 240P 20 kbps Completed 18:46, 23 October 2018 5.0 s
Streaming 240p (VP9) 20 kbps Completed 19:54, 20 December 2023 1.0 s
WebM 360P 303 kbps Completed 23:27, 11 November 2012 9.0 s
Streaming 144p (MJPEG) 136 kbps Completed 07:32, 10 November 2023 1.0 s

Metadata

This file contains additional information such as Exif metadata which may have been added by the digital camera, scanner, or software program used to create or digitize it. If the file has been modified from its original state, some details such as the timestamp may not fully reflect those of the original file. The timestamp is only as accurate as the clock in the camera, and it may be completely wrong.

Retrieved from "https://commons.wikimedia.org/w/index.php?title=File:Tonic-Shock-Induces-Detachment-of--Giardia-lamblia-pntd.0000169.s001.ogv&oldid=865060906"