File:Two hybrid assay.svg

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Overview of two-hybrid assay, checking for interactions between two proteins, called here Bait and Prey.
A. Gal4 transcription factor gene produces two domain protein (BD and AD) which is essential for transcription of the reporter gene (LacZ).
B,C. Two fusion proteins are prepared: Gal4BD+Bait and Gal4AD+Prey. None of them is usually sufficient to initiate the transcription (of the reporter gene) alone.

D. When both fusion proteins are produced and Bait part of the first interact with Prey part of the second, transcription of the reporter gene occurs.
Date
Source Own work
Author Anna
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Date/TimeThumbnailDimensionsUserComment
current17:45, 2 March 2010Thumbnail for version as of 17:45, 2 March 2010709 × 886 (86 KB)Contele de Grozavesti (talk | contribs)Tiny change in parentheses' location.
18:15, 9 October 2007Thumbnail for version as of 18:15, 9 October 2007709 × 886 (86 KB)Anna K. (talk | contribs)Converted to plain SVG with some objects converted to paths.
17:34, 9 October 2007Thumbnail for version as of 17:34, 9 October 2007709 × 886 (44 KB)Anna K. (talk | contribs){{Inkscape}} {{Information |Description=Two hybrid assay |Source=own work |Date=October 2007 |Author=Anna |other_versions= }} {{Translation possible}} Category:SVG Category:Diagrams in English

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