File:Viral-Transmission-Dynamics-at-Single-Cell-Resolution-Reveal-Transiently-Immune-Subpopulations-pgen.1005770.s002.ogv

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Viral-Transmission-Dynamics-at-Single-Cell-Resolution-Reveal-Transiently-Immune-Subpopulations-pgen.1005770.s002.ogv ‎(Ogg Theora video file, length 10 s, 718 × 468 pixels, 2.29 Mbps, file size: 2.84 MB)

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English: Time-lapse movie showing phage propagation throughout a single, exponentially growing, P22-free lineage from the moment its transient resistance disappears. An exponential phase population of LT2ΔpSLT/pALA2705 was infected with P22 Δint parS (MOI = 0.1) and grown in a semi-continuous culture for 5 hours before plating on an agar pad seeded with additional P22 parS. Please note that phage entry can occur with either P22 Δint parS already present in the culture or the added P22 parS. Analysis of 33 similar lineages revealed that under these conditions transient resistance on average lasted for ca. 12 generations, based on a doubling time of ca. 55 min in AB-glycerol media. Phase contrast images (showing the cells) and GFP signal (reporting the P22 parS chromosomes) are merged. A 5 μm scale bar is shown in the bottom right and the timestamp indicates time after plating cells for microscopy analysis.
Date
Source S1 Movie from Cenens W, Makumi A, Govers S, Lavigne R, Aertsen A (2015). "Viral Transmission Dynamics at Single-Cell Resolution Reveal Transiently Immune Subpopulations Caused by a Carrier State Association". PLOS Genetics. DOI:10.1371/journal.pgen.1005770. PMID 26720743. PMC: 4697819.
Author Cenens W, Makumi A, Govers S, Lavigne R, Aertsen A
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This file was transferred to Wikimedia Commons from PubMed Central by way of the Open Access Media Importer.
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Date/TimeThumbnailDimensionsUserComment
current03:30, 15 January 201610 s, 718 × 468 (2.84 MB)Open Access Media Importer Bot (talk | contribs)Automatically uploaded media file from Open Access source. Please report problems or suggestions here.

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VP9 360P 415 kbps Completed 17:27, 25 October 2018 6.0 s
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VP9 240P 192 kbps Completed 17:27, 25 October 2018 4.0 s
Streaming 240p (VP9) 192 kbps Completed 23:53, 21 December 2023 1.0 s
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Streaming 144p (MJPEG) 240 kbps Completed 01:50, 18 November 2023 1.0 s

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