File:Zebrafish Embryonic Notochord Development Inactivation of Pacsin 3 in zebrafish embryos.jpg
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| Description |
Figure 4. Inactivation of Pacsin 3 in zebrafish embryos. (A–D) Morphology of representative control (scrambled) or pacsin3 MO-injected 24 hpf embryos. Anterior is left. Bar = 250 mm. (E) Phenotypic quantitation of normal (blue), mild (white), or severely (black) affected embryos injected with control (n = 42) or 2 ng (n = 48), 3 ng (n = 53), 4 ng (n = 57), or 5 ng (n = 54) pacsin3 MO, or with both 5 ng pacsin3 and 5 ng p53 MOs (n = 61). (F–G) Close-up views of abnormal notochord (white arrowheads) and improperly structured somites (red arrowhead) in 24 hpf pacsin3 MO- injected embryos. Bar = 250 mm. (H–J) Representative lateral views of control or pacsin3 MO-injected 48 hpf embryos. Note lack of the yolk tube (arrow) even in mildly affected pacsin3 morphants. Bar = 250 mm. (K–M) Localization of ntla mRNA in typical control or pacsin3 MO-injected 24 hpf embryos. Anterior is up in K and M. Bar = 250 mm. (N–P) GFP fluorescence from injection of 25 pg GFP-Pacsin3D (N, O) or GFP (P) mRNA into embryos together with no (N) or 5 ng pacsin3 MO (O, P) at the one-cell stage. Groups of embryos still within the chorion at ,5 hpf and typical individual 24 hpf embryos show effective and selective silencing of the pacsin3 transcript. Bar = 250 mm. (Q–S) Gross morphology of 3 ng pacsin3 MO-injected embryos co-injected with 50 pg GFP (Q) or 50 pg GFP and either 25 pg D. rerio Pacsin2 (R) or D. melanogaster Syndapin (S) mRNA. Bar = 250 mm |
| Date | |
| Source | Edeling MA, Sanker S, Shima T, Umasankar PK, Ho¨ ning S, et al. (2009) Structural Requirements for PACSIN/Syndapin Operation during Zebrafish Embryonic Notochord Development. PLoS ONE 4(12): e8150. doi:10.1371/journal.pone.0008150 https://d-scholarship.pitt.edu/13299/1/Structural_Requirements.pdf |
| Author | James Keen, Thomas Jefferson University, United States of America |
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ß 2009 Edeling et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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| current | 10:25, 27 April 2024 | | 760 × 925 (575 KB) | Rasbak (talk | contribs) | {{Information |description=Figure 4. Inactivation of Pacsin 3 in zebrafish embryos. (A–D) Morphology of representative control (scrambled) or pacsin3 MO-injected 24 hpf embryos. Anterior is left. Bar = 250 mm. (E) Phenotypic quantitation of normal (blue), mild (white), or severely (black) affected embryos injected with control (n = 42) or 2 ng (n = 48), 3 ng (n = 53), 4 ng (n = 57), or 5 ng (n = 54) pacsin3 MO, or with both 5 ng pacsin3 and 5 ng p53 MOs (n = 61). (F–G) Close-up views of abnor... |
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